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目的研究6-甲基-11-(4-二甲基氨基苄烯)-7,8,9,10-四氢-1-氢吲哚[1,2b]吲哒唑三氟甲磺酸盐对人脑胶质瘤U251细胞增殖,凋亡和细胞周期的影响。方法分别采用噻唑蓝(MTT)法检测U251细胞增殖活性、Hoechst33258荧光染色检测细胞凋亡形态、流式细胞仪检测细胞周期、Western blot检测细胞周期蛋白Cyclin D1和Cyclin B1的表达变化。结果 MTT法检测发现6-甲基-11-(4-二甲基氨基苄烯)-7,8,9,10-四氢-1-氢吲哚[1,2b]吲哒唑三氟甲磺酸盐能显著抑制U251细胞的生长,且呈浓度及时间依赖性,Hoechst33258荧光染色检测观察到细胞凋亡形态的改变,流式细胞仪检测发现细胞阻滞于G2/M期和S期,Western blot检测发现细胞周期蛋白Cyclin D1和Cyclin B1表达下调。结论 6-甲基-11-(4-二甲基氨基苄烯)-7,8,9,10-四氢-1-氢吲哚[1,2b]吲哒唑三氟甲磺酸盐对人脑胶质瘤U251细胞增殖有明显的抑制作用,并能诱导其发生凋亡。其凋亡作用可能与G2/M期和S期阻滞有关。
Aim To study the effect of 6-methyl-11- (4-dimethylaminobenzyl) -7,8,9,10-tetrahydro-1-indol [1,2b] indazole triflate Effects on proliferation, apoptosis and cell cycle of human glioma U251 cells. Methods The proliferative activity of U251 cells was detected by MTT assay. The apoptosis of U251 cells was detected by Hoechst33258 staining. The cell cycle was detected by flow cytometry. The expressions of Cyclin D1 and Cyclin B1 were detected by Western blot. Results MTT assay showed that 6-methyl-11- (4-dimethylaminobenzyl) -7,8,9,10-tetrahydro-1-indol [1,2b] Sulfonate significantly inhibited the growth of U251 cells in a dose- and time-dependent manner. Fluorescence staining with Hoechst 33258 detected morphological changes of apoptotic cells. Flow cytometry revealed that cells arrested in G2 / M phase and S phase, Western blot showed that the expressions of cyclin D1 and Cyclin B1 were down-regulated. Conclusion 6-Methyl-11- (4-dimethylaminobenzyl) -7,8,9,10-tetrahydro-1-indolo [1,2b] indazole triflate Human glioma U251 cell proliferation was significantly inhibited, and can induce apoptosis. Its apoptosis may be related to G2 / M phase and S phase arrest.