论文部分内容阅读
目的分别探讨低温(-20℃和-80℃)以及冻存时间对血清胰岛素(INS)稳定性的影响。方法分离胶真空采血管采集72份静脉血并分离血清,于-20℃及-80℃分别放置3、7、14d,使用微粒子化学发光免疫分析法测定其INS水平,并按INS水平分为低、中和高3组。除使用Bland-Altman分析法评估两种温度条件间,以及各时间点间INS水平的可比性,还将两个时间点INS水平差异率与INS的总变化极限(TCL)值进行比较,以进一步评估偏差的可接受性。结果在-20℃和-80℃条件下储存相同时间的标本,其INS水平具有很好的可比性;冻存后第3天的标本,Bland-Altman法显示其INS与即时水平无可比性,但3d后趋于稳定;相对于即时水平,多数血清标本冻存不同时间后INS降低幅度低于TCL值(低值组TCL为13.3%,中、高值组TCL为15.6%),变化幅度基本可被接受。结论 -20℃和-80℃保存血清标本,对防止INS被降解的效果相当;即使低温保存,血清INS也存在一定程度的降解,但如无法及时检测,低温保存仍为防止INS被降解的有效方法。
Objective To investigate the effects of low temperature (-20 ℃ and -80 ℃) and freezing time on serum insulin (INS) stability. Methods Seventy-two venous blood samples were collected from the sealants and sera were collected. The sera were stored at -20 ℃ and -80 ℃ for 3, 7 and 14 days, respectively. The level of INS was measured by chemiluminescence immunoassay. , And high in 3 groups. In addition to using Bland-Altman analysis to assess the comparability of INS levels between two temperature conditions and at various time points, INS level difference rates at two time points were compared with the total change in limit (TCL) value of INS to further Assess the acceptability of bias. Results The samples at the same time were stored at -20 ℃ and -80 ℃, and the INS level was well comparable. On the third day after storage, the Bland-Altman method showed that the INS was not comparable with the instantaneous level, However, after 3 days, the serum INS level was lower than the TCL value (TCL was 13.3% in the low value group and 15.6% in the middle and high value group), compared with the instantaneous level. The change range was basically Can be accepted. Conclusions Serum samples stored at-20 ℃ and -80 ℃ are equivalent to preventing the degradation of INS. Even if stored at low temperature, there is a certain degree of degradation of serum INS, but if it can not be detected in time, low-temperature storage is still effective in preventing the INS from being degraded method.