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目的了解新疆伊犁哈萨克自治州(伊犁州)当前流行的HIV毒株亚型种类、人群分布特征及相关危险因素,对利用核酸技术检测新发感染的方法进行初步评价并开展HIV病毒分子流行病学调查。方法应用套式聚合酶链反应(PCR)对289份HIV-1感染者淋巴细胞富集液进行RNA提取、扩增、测序、基因型分析和亚型确定。结果经检测获得基因型样本246份,其中CRF07_BC毒株243份,占98.8%;CRF01_AE毒株2份,占0.8%;B’毒株1份,占0.4%;核酸检测新发感染阳性扩增率为84.8%。结论 CRF07_BC仍然是当前伊犁州艾滋病病毒感染者的优势毒株,在HIV确认样本时,核酸检测新发感染的方法获得了较高的阳性扩增率。
Objective To understand the prevalence of HIV subtypes, population distribution and related risk factors in Yili Kazakh Autonomous Prefecture (Ili Prefecture) of Xinjiang Uygur Autonomous Region (Ili Prefecture), and to conduct preliminary evaluation of the methods for detection of new infections using nucleic acid and carry out molecular epidemiological investigation of HIV . Methods Totally 289 HIV-1-infected lymphocyte enriched RNAs were amplified by RNA polymerase (RNAi), amplified, sequenced, genotyped and subtype confirmed by nested polymerase chain reaction (PCR). Results A total of 246 genotypes were detected, of which 243 were CRF07_BC, accounting for 98.8%; 2 were CRF01_AE, accounting for 0.8%; 1 was B ’, accounting for 0.4% The rate was 84.8%. Conclusion CRF07_BC is still the predominant strain of HIV-infected people in Yili Prefecture. When HIV samples were confirmed, nucleic acid detection of new infections achieved a higher positive rate of amplification.