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目的:观察代谢型谷氨酸受体(mGluRs)激动药3,5-二羟基苯甘氨酸(DHPG)对大鼠海马CA1区穿通通路(PP通路)诱发长时程抑制的作用及机制。方法:制作Wistar大鼠海马组织脑片,分为DHPG组、DHPG+N-甲基-D-天冬氨酸(NMDA)受体拮抗药AP5组和DHPG+双脉冲刺激(PPR)组(n分别为8、9、8),各组加入相应药物及操作后采用电生理记录加药前、后的场电位的标准化后均值。结果:DHPG组、DHPG+AP5组、PPR组加药前、后场电位均值分别为1.0049±0.101、0.7904±0.02197(n=8,t=15,P<0.01),1.0224±0.0724、0.828±0.01475(n=9,t=15,P<0.01),0.9525±0.1691、1.0511±0.2934(n=8,t=5,P<0.01)。结论:DHPG可诱发大鼠海马长时程抑制,且其属于mGluRs依赖型而非NMDA受体依赖型,并通过突触后诱发所致。
AIM: To observe the effect and mechanism of 3,5-dihydroxyphenylglycine (DHPG), a metabotropic glutamate receptor (mGluRs) agonist, on the long-term inhibitory effect induced by the perinatal hippocampal CA1 region (PP pathway) in rats. Methods: The Wistar rat hippocampal slices were prepared and divided into DHPG group, DHPG + N-methyl-D-aspartate (NMDA) antagonist AP5 group and DHPG + PPR group 8,9,8). The normalized average values of the field potentials before and after the addition of the corresponding drugs and the electrophysiological records after the operation were recorded in each group. Results: The average values of the potential before and after DHPG, DHPG + AP5 and PPR were 1.0049 ± 0.101,0.7904 ± 0.02197 (n = 8, t = 15, P <0.01), 1.0224 ± 0.0724 and 0.828 ± 0.01475 (n = 9, t = 15, P <0.01), 0.9525 ± 0.1691, 1.0511 ± 0.2934 (n = 8, t = 5, P <0.01). Conclusion: DHPG can induce long-term inhibition of hippocampus in rats, and it belongs to mGluRs-dependent but not NMDA-dependent and is induced by postsynaptic induction.