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用含不同浓度NaCl的Hoagland溶液培养 6天龄大麦幼苗 ,同时向心叶滴加 5 0 μmol/LABA (脱落酸 )和叶片饲喂14 C Glu (谷氨酸 )。结果显示 ,5 0～ 1 0 0mmol/LNaCl促进了14 C Glu向质膜蛋白掺入 ,5 0～ 2 0 0mmol/LNaCl促进了14 C Glu向液泡膜蛋白的掺入 ,其中对向根系液泡膜蛋白掺入的促进作用更显著。 5 0mmol/LNaCl对14 C Glu向叶片类囊体膜蛋白的掺入没有影响 ,超过 5 0mmol/L的NaCl则抑制掺入。 30 0mmol/L以内的NaCl对14 C Glu向根系线粒体膜蛋白掺入的影响较小。盐胁迫下 5 0 μmol/LABA可明显促进14 C Glu向质膜、液泡膜和类囊体膜蛋白的掺入 ,对向线粒体膜蛋白的掺入没有影响。蛋白酶活性随处理盐浓度的上升而上升 ,5 0 μmol/LABA处理能轻微降低蛋白酶活性的上升
Six-day-old barley seedlings were cultured in Hoagland solution containing different concentrations of NaCl. Fifty μmol / L ABA (abscisic acid) was added to the leaves of the heart and 14 C Glu (glutamate) was fed on the leaves. The results showed that 50 ~ 100 mmol / L NaCl promoted the incorporation of 14 C Glu into the plasma membrane protein and 50 ~ 200 mmol / L NaCl promoted the incorporation of 14 C Glu into the vacuolar membrane protein. Promote the role of protein incorporation more significant. 5 0 mmol / L NaCl had no effect on the incorporation of 14 C Glu into the thylakoid membrane protein, while more than 50 mmol / L NaCl inhibited the incorporation of 14 C Glu into the leaf thylakoid membrane protein. The effects of NaCl at 30 mmol / L on the incorporation of 14 C Glu into root mitochondrial membrane protein were insignificant. Under salt stress, 50 μmol / L LABA could significantly promote the incorporation of 14 C Glu into plasma membrane, vacuolar membrane and thylakoid membrane proteins, but had no effect on the incorporation of mitochondrial membrane proteins. Protease activity increased with increasing salt concentration, and 50 μmol / L LABA slightly decreased the protease activity