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运用随机扩增多态DNA(RAPD)技术对发生于我国 1 3省区 1 6种松树和其它 2种针叶树上的松枯梢病菌 (Sphaeropsissapinea)的 55个菌株进行基因组DNA多态性分析。用 1 7个随机引物经PCR扩增共得到 2 0 0个RAPD标记 ,其中 98 5%具有多态性。UPGMA聚类分析确定了供试菌株间的亲缘关系 ,将 55个菌株分为 3个类群。各菌株间的差异与其寄主种类无明显关系 ,与其地理来源在某些类群间有一定联系 ,但在大多数菌株间相关趋势不明显。
Fifty-five strains of sphaeropsissapinea occurring in 16 species of pine and 13 species of conifers in 13 provinces of China were analyzed for genomic DNA using random amplified polymorphic DNA (RAPD) technique. A total of 200 RAPD markers were obtained by PCR amplification with 17 random primers, of which 98 5% were polymorphic. UPGMA cluster analysis identified the genetic relationship between the strains tested, 55 strains were divided into three groups. The differences between the strains were not significantly related to the host species, and some sources were related to their geographical origin, but the correlation trend was not obvious between most strains.