冰冻切片作为一种快速诊断方法,早已广泛应用于常规病理诊断。冰冻切片诊断的准确性与高质量的冰冻切片密切相关,但常规染色方法染出的冰冻切片在显微镜下观察不够清晰、透明,往往给病理科医生在诊断上造成一定的难度。 在长期工作中,我们发现影响冰冻切片染色质量的主要原因是活体组织中含有一定 Frozen section as a rapid diagnostic method, has long been widely used in routine pathological diagnosis. The accuracy of frozen section diagnosis is closely related to high-quality frozen sections. However, the frozen sections stained by conventional staining methods are not clear and transparent under the microscope, which often leads to pathologists in diagnosing certain difficulties. In long-term work, we found that the main reason affecting the quality of frozen section staining is that living tissue contains certain