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目的:研究表达小鼠白细胞介素21(mIL-21)的Sp2/0细胞与用Sp2/0细胞预先免疫的小鼠淋巴细胞体外共培养,是否对预致敏淋巴细胞增殖及功能有影响。方法:获取灭活Sp2/0细胞免疫的小鼠淋巴细胞,在mIL-2存在的条件下,以mIL-21转染的Sp2/0细胞为刺激细胞,用流式细胞术检测CFSE标记的淋巴细胞增殖和7-AAD标记的细胞毒活性;用ELISpot法确定分泌IFN-γ的淋巴细胞数量。结果:转染mIL-21的Sp2/0细胞对预致敏的淋巴细胞增殖有明显影响,活化的淋巴细胞对靶细胞的杀伤率(39.57%±4.72%)与对照组(23.18%±2.94%)相比有较大的提高(P<0.05),且分泌IFN-γ的细胞数量明显增加。活化增殖后的淋巴细胞回输至环磷酰胺预处理的小鼠,能延长小鼠的成瘤时间。结论:表达mIL-21的Sp2/0细胞可有效促进肿瘤抗原特异性淋巴细胞活化及增殖,并增强其对肿瘤细胞的杀伤功能。
OBJECTIVE: To investigate whether in vitro co-culture of mouse Sp2 / 0 cells expressing mouse interleukin-21 (mIL-21) and mouse lymphocytes pre-immunized with Sp2 / 0 cells affects the proliferation and function of primed lymphocytes. Methods: Splenocytes from mice immunized with Sp2 / 0 cells were obtained and stimulated with mIL-21-transfected Sp2 / 0 cells in the presence of mIL-2. Flow cytometry was used to detect CFSE-labeled lymphocytes Cell proliferation and 7-AAD labeled cytotoxic activity; ELISpot method to determine the number of IFN-γ-secreting lymphocytes. Results: Sp2 / 0 cells transfected with mIL-21 significantly affected the proliferation of pre-sensitized lymphocytes. The killing rate of activated lymphocytes to target cells (39.57% ± 4.72%) and control group (23.18% ± 2.94% ) (P <0.05), and the number of cells secreting IFN-γ significantly increased. The activated lymphocytes were transfused into cyclophosphamide pretreated mice, which could prolong the tumorigenic time of mice. Conclusion: Sp2 / 0 cells expressing mIL-21 can effectively promote tumor antigen-specific lymphocyte activation and proliferation, and enhance its killing effect on tumor cells.