论文部分内容阅读
用含0.01%的二乙基亚硝胺(DEN)水溶液作诱癌剂喂饲雄性SD 大鼠.在诱癌期间,分期取鼠肝进行病理和γ-谷氨酰转肽酶(γ-GT)及非特异性酯酶(NSE)组化检查,并用等电点聚焦电泳(IEF)观察肝匀浆中NSE 同工酶谱的变化。实验表明,从第2周起。肝组织中γ-GT活性逐渐增强,分布由小胆管转向肝细胞胞浆内。NSE 活性自第4周后开始进行性减弱,在肝癌细胞内活性消失.NSE 同工酶谱的变化早于酶组织化学示肝内NSE 活性变化。自第4周起,部份鼠肝匀浆在pH6.93～7.72的同工酶活性减弱或消失,带瘤匀浆中这种变化尤为明显.
Male SD rats were fed with an aqueous solution containing 0.01% diethylnitrosamine (DEN) as a carcinogen. During the induction of cancer, livers from rats were used for pathological examination and γ-glutamyl transpeptidase (γ-GT) was used. ) and non-specific esterase (NSE) histochemical examination, and isoelectric focusing (IEF) was used to observe the changes of NSE isoenzyme spectrum in liver homogenate. Experiments show that from the 2nd week. The γ-GT activity in liver tissue gradually increased, and the distribution turned from a small bile duct to the cytoplasm of liver cells. NSE activity began to decline after the fourth week and disappeared in the liver cancer cells. Changes in the NSE isoenzyme spectrum earlier than the enzyme histochemistry showed changes in the liver NSE activity. From the 4th week, the isoenzyme activity of some mouse liver homogenates at pH 6.93-7.72 weakened or disappeared. This change was especially evident in tumor-bearing homogenates.