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酯酶可以帮助血细胞分类,尤其在白血病细胞的分类中用途更大,但常规的细胞化学方法分类白血病细胞时有程度不同的主观性。等电聚焦技术分辨力很高,可将差别极小的酯酶同工酶分开,而各种血细胞都有酯酶,且其同工酶各具特点,所以应用这种技术可以更客观地分类血细胞。本文主要阐述各种正常血细胞、白血病细胞、淋巴细胞和培养的血细胞株酯酶同工酶等电聚焦。一、正常血细胞不同类型正常血细胞酯酶同工酶不同,在相同实验条件下,同类细胞同工酶带数量、各带反应强度和各自的等电点(PI)相同,等电点相差最多不超过0.2pH单位。
Esterases can help classify blood cells, especially in the classification of leukemia cells, but conventional cytochemical methods classify leukemia cells with varying degrees of subjectivity. Isoelectric focusing technology has a high resolution and can separate the esterase isoenzymes with very little difference. The various blood cells have esterases and their isozymes have different characteristics. Therefore, the application of this technology can be more objectively classified. blood cells. This article mainly describes the electro-focusing of esterase isoenzymes in various normal blood cells, leukemia cells, lymphocytes and cultured blood cell strains. First, the normal blood cells of different types of normal blood cells esterase isoenzymes are different, under the same experimental conditions, the number of isoenzyme bands in the same type of cells, the intensity of each band and their respective isoelectric point (PI) the same, the difference in isoelectric point is not the most More than 0.2 pH units.