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目的优化人参皂苷Rg3微乳的处方,并对其质量进行评价。方法采用HPLC测定人参皂苷Rg3在油相、表面活性剂和助表面活性剂中的溶解度,通过伪三元相图和星点设计-效应面法优选处方。结果油酸乙酯、EL35-PEG400与水的最佳质量比为8.25∶12∶4.75∶75,平均粒径为60.50 nm,Zeta电位为-15.63 m V。微乳经4000 r·min-1离心10 min后无分层现象。结论该工艺方法简便、稳定可行,适用于人参皂苷Rg3微乳的制备。
Objective To optimize the prescription of ginsenoside Rg3 microemulsion and evaluate its quality. Methods The solubility of ginsenoside Rg3 in oil phase, surfactant and cosurfactant was determined by HPLC. The prescription of ginsenoside Rg3 was optimized by pseudo-ternary phase diagram and star-point design-response surface method. Results The optimum mass ratio of ethyl oleate, EL35-PEG400 and water was 8.25:12:4.75:75, the average particle size was 60.50 nm and the zeta potential was -15.63 mV. After microemulsion centrifugation at 4000 r · min-1 for 10 min, no delamination occurred. Conclusion The method is simple, stable and feasible, and is suitable for the preparation of ginsenoside Rg3 microemulsion.