Isolation of Leptospirillum ferriphilum by single-layered solid medium

来源 :中南工业大学学报(英文版) | 被引量 : 0次 | 上传用户:peterpan984
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium.Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining,scanning electron microscope(SEM) and transmission electron microscope (TEM). The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum. Additionally,physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 ℃,respectively. The culture of it is used to leach a complex concentrate chalcopyrite, the leaching efficiencies of copper and iron are 1.93% and 13.74%, respectively, and it is more effective than the A.ferrooxidans culture in the leaching of the complex concentrate chalcopyrite.
其他文献
设计工作坊是一种项目式、主动式的创新实践教学方式,是隐性知识向显性知识转化的重要场所,一般由经验丰富的设计师或研究者主持,通过互动讨论、方案演示等环节实现设计创意
Microbial community diversities in the drainage from three mines (Dexing Copper Mine, Qibaoshan Copper Mine and Yaogangxian Tungsten Mine, China) were analyzed
2012年,笔者与同事们一起作南京大屠杀幸存者老年生活现状调查,完成后先后在好几个全国学术会议上作报告。在我们调查的39位老年人中,年龄最大的95岁,最小的75岁,平均年龄85.
2015年1月19日,上海股市综合指数暴跌7.7%,创7年最大单日跌幅,盘中最大跌幅逾8%;深证成指跌6.61%;金融股全部跌停,权重股近乎集体跌停;股指期货主力合约跌停。诱因据说是受到政府部门的联合打压,具体而言,上周五,证监会通报券商融资类业务现场检查情况,银监会发布《商业银行委托贷款管理办法(征求意见稿)》,同时央行货币政策司官员又撰文称要防止过度“放水”。   于是乎,这次的市场震荡为我
#
期刊
On the basis of energy and shape method for the determination of the valence bond (VB) structures of crystal, the valence bond structure of titanium is redeterm
去看望一位老者,离休20多年,著书作画,成果颇丰,见人总是笑呵呵的,好像从没烦心事似的。我询问他是怎样养生的。他笑着说,人生就好比一卷手纸,越到后面用得越快。离休后生命已进入倒计时,我就珍惜每一天,清晨一睁开眼就告诉自己:“今天是最好的一天!”我和他攀谈起来,受益匪浅。最近看书,发现美国98岁的著名医学家奥斯勒,有人曾问他长寿的秘诀,他只说了四个字:“今天最好!”由此看来,“今天最好”亦是一条养生
由国家农业部等部门主办,在中央电视台第七套节目中播出的《全国农民春节晚会》已经连续举办8年了。农民春晚已成为亿万农民过春节不可或缺的文化大餐。广大农民观众对于这个
全国海关将从2002年1月1日,实施新的审定进出口货物完税价格办法。海关总署表示,遵循客观、公平、统一的估价原则,依据新办法审定进出口货物的完税价格。进口货物的完税价格,由海关以该