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多肽激素的~(125)碘化标记对放射免疫分析、放射性受体分析、细胞膜抗原及免疫学研究均有重要意义。1963年Greenwood等创用氯胺T~(125)碘化法,国内外目前仍沿用,但氯胺T氧化作用较强,常使标记的多肽激素的生物活性和免疫活性受到损伤。七十年代中期国外学者进行乳过氧化物酶(Lactoproxidase,简称LPO)酶促~(125)碘化法,由于此法作用温和,故能使标记的多肽激素保持较好的生物及免疫活性,提高了标记激素的保存稳定性。我室在1972～1978年中应用氯胺T法~(125)碘化多肽激素实践的基础上,于1978～1981年起先后完成五种多肽激素的酶促~(125)碘化,并结合国内目前供应的~(125)碘
The ~ (125) iodinated labeling of polypeptide hormones is of great importance to radioimmunoassay, radioactive receptor analysis, cell membrane antigen and immunological studies. In 1963, Greenwood et al. Used chloramine T ~ (125) iodide method, which is still used at home and abroad. However, the oxidation of chloramine T is strong, which often impairs the bioactivity and immunological activity of labeled peptide hormones. In the mid seventies, foreign scholars carried out the enzymatic 125 iodination method of lactoperoxidase (LPO). Because of the mild action of this method, the labeled polypeptide hormone can maintain good biological and immunological activity, Improves the storage stability of labeled hormones. Based on the practice of chloramine T method and 125 iodinated polypeptide hormone in our lab from 1972 to 1978, enzymatic 125 iodination of five polypeptide hormones was completed from 1978 to 1981 and combined Domestic supply of ~ (125) iodine