致半滑舌鳎烂尾病的创伤弧菌的分离鉴定及中草药对其抑菌效果研究

来源 :中国预防兽医学报 | 被引量 : 0次 | 上传用户：czjjay

【摘要】

：

为检测秦皇岛市昌黎县某渔场养殖的致半滑舌鳎烂尾病的致病原,检测分离菌对抗生素的敏感性以及中草药对其的体外抑菌效果,本研究从患病鱼尾部肌肉分离纯化到一株细菌,采用TCBS鉴定培养基培养,结果可见绿色菌落,边缘整齐,表面光滑,轻微凸起;经革兰氏染色后镜检可见,分离菌为革兰氏阴性菌,两端钝圆,形状似月牙;经ATB全自动微生物鉴定仪判定,分离菌为创伤弧菌,符合率为99%;通过16S rDNA序列分析进一步鉴定分离菌为创伤弧菌,命名为CLVV-1。采用动物回归试验检测分离菌株的致病性,结果显示CLVV-1菌株可致半

【作者】

：

刘勃兴崔舒畅任海靳晓敏韩红生史秋梅高桂生

【机构】

：

河北科技师范学院河北省预防兽医学重点实验室,秦皇岛粮丰海洋生态技术开发有限公司

【出处】

：

中国预防兽医学报

【发表日期】

：

2021年5期

【关键词】

：

半滑舌鳎烂尾病创伤弧菌耐药性中草药

【基金项目】

：

河北省科技厅项目(2028701Z),河北科技师范学院海洋专项(2018HU014),河北科技师范学院海洋专项(2018HU007)。

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

其他文献

番鸭源鹅细小病毒VP2病毒样颗粒制备及免疫原性测定

为制备杆状病毒表达系统表达的番鸭源鹅细小病毒(MDGPV)VP2蛋白形成的病毒样颗粒(VLPs),并测定其免疫原性,本研究通过PCR扩增得到MDGPV PT株VP2基因,通过杆状病毒表达系统构建了表达MDGPV VP2的重组杆状病毒MDGPV-rBV。以MOI 5的MDGPV-rBV感染Sf9细胞后,通过间接免疫荧光试验(IFA)检测结果显示,MDGPV-rBV感染的细胞出现亮绿色荧光;western blot检测VP2蛋白,结果显示在约65 ku处出现特异性条带,表明VP2蛋白正确表达。收集MDGPV-

期刊

番鸭源鹅细小病毒病毒样颗粒免疫原性番鸭

Visible Light-Promoted Sulfoxonium Ylides Synthesis from Aryl Diazoacetates and Sulfoxides

A visible light-promoted reaction of donor/acceptor diazoalkanes with sulfoxides towards the synthesis of synthetically useful sulfoxonium ylides was reported.The reaction occurred under sole visible light irradiation without the need of any transition-me

期刊

SulfoxoniumylidesSyntheticmethodsCARBENEVisibleLightDiazocompounds

Single-Component Chemical Nose with a Hemicyanine Probe for Pattern-Based Discrimination of Metal Io

Main observation and conclusionrnWe report here a chemical nose utilizing the nonspecificity of a hemicyanine dye(probe P)containing three acetates and one depr

期刊

Chemical noseCationsSensorsUV/Vis spectroscopyPrinciple component analysis

Target Extension-Activated DNA Walker on Nanoparticles for Digital Counting-Based Analysis of MicroR

MicroRNAs(miRNAs),especially exosomal miRNAs,are promising noninvasive biomarkers in early-stage cancer diagnosis and disease treatment monitoring.However,their precise and sensitive quantification remains challenging due to their small size and low abund

期刊

RNArecognitionDNAcleavageDigitalcountingTotalinternalreflectionfluoresc

Antiosteoporotic Tetrahydroxanthone Dimers from Aspergillus brunneoviolaceus FB-2 Residing in Human

A complex community of human-associated microorganisms plays diversely important roles in maintaining health,and the metabolites help to define whether a particular human-associated microbe is a friend or a foe.This work shows that Aspergillus brunneoviol

期刊

AspergillusbrunneoviolaceusGut-associatedfungusNaturalproductsStructureel

社会治理、社会地位与社会公平感知

文章基于中国综合社会调查CGSS2015的相关数据,选取社会治理和社会地位作为解释变量,选取社会公平感知作为被解释变量,运用二元分类Logit回归模型依次测度各自变量对社会公平感知的影响效应。结果表明:社会治理对社会公平感知具有显著正向影响。在考虑控制变量的条件下,每增加1个单位的社会治理,社会公平感知能够提升83.9%;社会地位对社会公平感知具有显著正向效应,在考虑控制变量的条件下,社会地位每提升1个层次,个体的社会公平感知就提高66.7%。因此,应当重视社会治理,提升社会治理层次与质量,同时尊重地位差

期刊

社会治理社会地位社会公平感知LOGIT回归模型

Design of Polymers for Intracellular Protein and Peptide Delivery

Cytosolic protein delivery techniques are of great importance for cell biology,biotechnology and protein drug development.The design of carriers with robust efficiency in cytosolic protein delivery is challenging.This account provides a progress report of

期刊

POLYMERSIntracellualrproteindeliveryPEPTIDESCancertherapyDENDRIMERS

Rational Design and Development of Low-Price,Scalable,Shelf-Stable and Broadly Applicable Electrophi

Main observation and conclusionrnThe development of two highly reactive electrophilic trifluoromethylating reagents(trifluoromethyl)(4-nitrophenyl)bis(carbometh

期刊

FluorineTrifluoromethylYlidesElectrophilicRadicals

Selective Recognition of Quaternary Ammonium Ions by Structurally Flexible Cages

The binding behaviors of three structurally similar and flexible cages to a series of quaternary ammonium ions have been systematically studied.The cage with phenyl sidewalls is new;moreover,its structure and conformational states have been characterized

期刊

MacrocyclesMolecularrecognitionHost-guestsystemsSupramolecularchemistryTH

WO3 Inversce Opal Photonic Crystals:Unique Property,Synthetic Methods and Extensive Application

Tungsten trioxide inverse opal photonic crystals(WO₃ IOPCs)not only possess the intrinsic properties of WO₃/but also own the unique characteristics of lOPCs structure.The evolution of preparation method of WO₃ IOPCs has be

期刊

WO_(3)InverseopalTemplatesynthesisSensorsPHOTOCATALYSISElectrochromic

致半滑舌鳎烂尾病的创伤弧菌的分离鉴定及中草药对其抑菌效果研究

与本文相关的学术论文