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云南三七产量高、质量好,是我国中药三七的主要来源。祖国医学关于三七生血、补血作用早有记载,关于其生血补血作用的机理研究报道也不少,但采用造血干细胞培养方法探讨其作用机理的文献尚未见到。我室与云南药物研究所协作,进行了三七皂甙对造血干细胞作用的研究。一、材料与方法(一)培养方法: 1、培养皿的准备:采用国产30mm直径的微量玻璃培养皿。用前先用肥皂水及自来水冲洗,烤干后用洗液浸泡24小时,然后再清洗并过蒸馏水,实验前一天经过150℃烤箱干热消毒后备用。2、小鼠骨髓细胞悬液的制备:取我室自养的日本ICR/JCL纯系小鼠三只,各取
Yunnan Sanqi has a high yield and good quality, and it is the main source of Chinese medicine Sanqi. The research on the role of blood and blood in Sanqi is well documented in the motherland medicine. There are also many reports on the mechanism of blood-enriching blood, but the literature on the mechanism of hematopoietic stem cell culture has not yet been seen. In collaboration with the Yunnan Institute of Materia Medica, our lab conducted a study of the effects of Panax Notoginseng on hematopoietic stem cells. I. Materials and Methods (I) Culture methods: 1. Preparation of Petri dishes: Domestically produced 30 mm diameter micro glass dishes were used. Rinse with soapy water and tap water before use, dry it and soak it with lotion for 24 hours, and then wash it with distilled water. The oven is dry and heat-disinfected at 150°C the day before the test. 2. Preparation of Mouse Bone Marrow Cell Suspension: Take three ICR/JCL pure mouse strains from our department’s autotrophy.