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实验采用了小鼠由细胞介导的免疫反应——二硝基氟苯(DNFB)所致接触超敏性和耐受性的动物摸型,以耳肿(Ear swelling)作为测量指标,观察了乙胺嘧啶对DNFB所致C_(57)小鼠接触超敏性和耐受性的影响。实验表明,乙胺嘧啶对适宜剂量DNFB致敏造成的接触超敏反应无影响。但乙胺嘧啶在致敏当天腹腔注射给予,能逆转由于大剂量DNFB致敏造成的免疫抑制,但其逆转程度不超过适宜剂量DNFB致敏造成最强免疫反应程度。在致敏当天给予乙胺嘧啶也能逆转由于静注DNFB耐受原造成的免疫耐受。结果提示,乙胺嘧啶逆转受抑免疫反应的作用不应称为“免疫增强”作用,拟称为“免疫逆转”作用为妥。乙胺嘧啶逆转受抑免疫反应和免疫耐受的机理可能是对抗原激活的抑制性T细胞(Ts)有选择性的抑制作用。乙胺嘧啶有可能作为免疫调节剂在科研和临床上加以应用。本文对C_(57)小鼠的接触超敏性和耐受性进行探讨,摸索了适宜致敏剂量和致耐剂量,并对动物摸型的作用和机理进行了讨论。
In this study, mice were exposed to hypersensitivity and tolerability induced by dinitrofluorobenzene (DNFB), a cell-mediated immune response. Ear swelling was used as a measure to observe Effect of pyrimethamine on contact hypersensitivity and tolerability in DNFB induced C57 mice. Experiments showed that pyrimethamine did not affect the contact hypersensitivity caused by DNFB sensitization at the appropriate dose. However, pyrimethamine was administered intraperitoneally on the day of sensitization, reversing the immunosuppression caused by the sensitization of large doses of DNFB, but its extent of reversal did not exceed the optimal dose for DNFB sensitization resulting in the strongest immune response. Pyrimethamine on the day of sensitization also reversed immune tolerance due to intravenous DNFB tolerance. The results suggest that the role of pyrimethamine in reversing the suppressed immune response should not be referred to as an “immune enhancement” effect, which is to be referred to as an “immune reversal” effect. The mechanism by which pyrimethamine reverses suppressed immune response and immune tolerance may be a selective inhibition of antigen-activated suppressive T cells (Ts). Pyrimethamine may be used as an immunomodulator in both scientific and clinical applications. In this paper, we investigated the contact hypersensitivity and tolerance of C57 mice and explored suitable sensitizing doses and tolerant doses, and discussed the action and mechanism of animal models.