目的:以卵巢组织中细胞色素C、线粒体Ca2+和性激素水平为观察指标,观察灵芝孢子粉对2型糖尿病大鼠卵巢组织的保护作用。方法:实验于2006-06/08在佳木斯大学黑龙江省小儿神经康复重点实验室完成。①实验对象:Wistar雌性大鼠50只,随机分为对照组10只,2型糖尿病模型组20只,灵芝孢子粉组20只。②实验方法:大鼠禁食16～18h,自由饮水,模型组及灵芝孢子粉组大鼠经尾静脉一次性按25mg/kg注射2%链脲佐菌素,正常对照组尾静脉注射等量柠檬酸钠-柠檬酸缓冲液。正常饮食喂养2周后,进行糖耐量试验,模型组和灵芝组以糖耐量异常者保留,余去除,并改喂高脂高糖饮食,灵芝孢子粉组另加灵芝孢子粉[250mg/(kg·d)]持续10周,实验结束前一天再次做糖耐量试验。③实验评估:实验结束后,各组动物均禁食12h,乙醚吸入浅麻醉,内眦静脉取血待测血清胰岛素。解剖出卵巢,待测线粒体细胞色素C、线粒体Ca2+含量。结果:选用Wistar雌性大鼠50只,结果分析数量每组8只。①葡萄糖耐量试验:实验开始及实验12周时,模型组大鼠均表现出糖耐量异常的变化(P<0.05),1h血糖增高显著(P<0.05)达到高峰,2h血糖仍较高;对照组大鼠30min血糖达到峰值,2h血糖基本恢复正常。②血清胰岛素和卵巢性激素水平:模型组血清胰岛素明显升高(P<0.05)。而灵芝孢子粉组与对照组比较差异无显著性。模型组雌二醇含量明显降低(P<0.05),卵胞刺激素含量、睾酮含量明显升高(P<0.05),而灵芝孢子粉组与对照组比较差异无显著性。③胞浆细胞色素C、线粒体细胞色素C、线粒体钙变化:模型组卵巢组织胞浆细胞色素C含量明显升高(P<0.05),模型组卵巢组织线粒体细胞色素C含量明显降低(P<0.05),模型组卵巢组织线粒体钙含量明显降低(P<0.05);而灵芝孢子粉组与对照组比较差异均无显著性。结论:灵芝孢子粉可降低糖尿病大鼠血清胰岛素、卵巢组织卵胞刺激素、睾酮、胞浆细胞色素C含量,调节卵巢组织雌二醇、线粒体细胞色素C、钙含量,对糖尿病大鼠卵巢组织具有一定保护作用。 Objective: To observe the protective effect of Ganoderma lucidum spores powder on ovarian tissues of type 2 diabetic rats by using cytochrome C, mitochondrial Ca2+, and sex hormone levels as indices. METHODS: The experiment was performed at the Key Laboratory of Pediatric Nerve Rehabilitation at Jiamusi University in Heilongjiang Province from June 2006 to August 2008. 1 Subjects: 50 Wistar female rats were randomly divided into control group 10, type 2 diabetic model group 20, and ganoderma spore powder group 20. 2 Experimental methods: Rats fasted for 16-18h, free drinking water, rats in model group and Ganoderma lucidum spore powder group were injected with 2% streptozotocin at a dose of 25mg/kg once via tail vein, and normal control group was injected equally with tail vein. Sodium citrate-citrate buffer. Two weeks after the normal diet, glucose tolerance test was performed. The model group and Ganoderma lucidum group were preserved with abnormal glucose tolerance, and the rest were removed, and were fed a high fat and high sugar diet. Ganoderma lucidum spore powder group was added with Ganoderma lucidum spore powder[250mg/(kg) d)] For 10 weeks, perform the glucose tolerance test again the day before the end of the experiment. 3 Experimental evaluation: After the end of the experiment, animals in each group were fasted for 12 hours. Ether was inhaled by light anesthesia. Intravenous venous blood was taken to measure serum insulin. The ovaries were dissected and the mitochondrial cytochrome C and mitochondrial Ca2+ contents were measured. RESULTS: A total of 50 female Wistar rats were used and the results were analyzed in 8 animals in each group. 1 Glucose tolerance test: At the beginning of the experiment and at 12 weeks of experiment, the rats in the model group all showed changes in glucose tolerance (P<0.05). The 1h blood glucose increased significantly (P<0.05) reached the peak, and 2h blood glucose was still higher; In group rats, the blood glucose reached its peak at 30min, and blood glucose returned to normal at 2h. 2 Serum insulin and ovarian sex hormone levels: In the model group, serum insulin was significantly increased (P<0.05). There was no significant difference between the Ganoderma lucidum spore powder group and the control group. The content of estradiol in the model group was significantly lower (P<0.05), and the content of stimulating hormone and testosterone were significantly increased (P<0.05). There was no significant difference between the ganoderma spore powder group and the control group. 3 Changes of cytoplasmic cytochrome C, mitochondrial cytochrome C, and mitochondrial calcium: cytoplasmic cytochrome C content in ovarian tissue of the model group increased significantly (P<0.05), and cytochrome C content of mitochondria in the ovarian tissue of model group decreased significantly (P<0.05). ) The mitochondrial Ca content in the ovarian tissue of the model group was significantly decreased (P<0.05). There was no significant difference between the Ganoderma lucidum spore powder group and the control group. Conclusion: Ganoderma lucidum spore powder can reduce serum insulin, ovarian tissue ovulation, testosterone, cytoplasmic cytochrome C content in diabetic rats, regulate ovarian tissue estradiol, mitochondrial cytochrome C, and calcium levels, and has an effect on the ovarian tissue of diabetic rats. Certain protection.