论文部分内容阅读
目的检测苯并(a)芘代谢产物反式二羟环氧苯并芘(反式-BPDE)及结晶型硫化镍(NiS)诱发永生化人支气管上皮细胞系(16HBE)恶性转化过程中线粒体DNA控制区序列变化。探讨线粒体DNA控制区在环境致癌物苯并(a)芘及硫化镍致癌作用中是否存在靶分子序列。方法应用银染PCR-单链构向多态性(SSCP)方法及测序方法分析恶变细胞中线粒体DNA高变区的序列变化。结果经反式-BPDE和NiS诱导恶变的人支气管上皮细胞系线粒体高变区均未发现异常改变。结论线粒体基因控制区可能并非是化学致癌物诱发肺癌的靶序列。
Objective To detect mitochondrial DNA (mtDNA) during the malignant transformation of immortalized human bronchial epithelial cell line (16HBE) induced by trans-BPDE (metabolites of benzo (a) pyrene and crystalline nickel sulfide Control area sequence changes. To investigate whether mitochondrial DNA control region in the carcinogen of environmental carcinogens benzo (a) pyrene and nickel sulfide carcinogenic role in the existence of target sequence. Methods The sequence variation of mitochondrial DNA hypervariable region in malignant cells was analyzed by silver staining PCR - single strand conformation polymorphism (SSCP) and sequencing. Results No abnormal changes were observed in the mitochondrial hypervariable regions of human bronchial epithelial cells induced by trans-BPDE and NiS. Conclusion Mitochondrial gene control regions may not be the target of chemical carcinogens induced lung cancer.