目的　构建GFP与H 2Kb 融合基因 ,并分析融合基因在活细胞中的表达。方法　应用基因工程技术构建H 2Kb 与绿色荧光蛋白 (GFP)的融合基因。RT PCR和Western印迹分析基因表达 ;激光共聚焦荧光显微镜观察活细胞内荧光布局。结果　RT PCR和Western印迹分析表明融合基因获得了正确表达。激光共聚焦荧光显微镜观察结果表明 ,Kb GFP融合分子象天然Kb 分子一样表达在细胞膜表面 ,且胞外区域可被Kb 分子特异性单克隆抗体所识别 ,表明Kb GFP融合分子可在细胞内正确折叠和装配。Kb GFP融合基因的瞬间和稳定表达均获得了相同结果。结论　Kb GFP融合蛋白具有GFP的自发荧光特性 ,且不影响Kb 分子在细胞内的正确表达。 Objective To construct the fusion gene of GFP and H 2Kb and analyze the expression of fusion gene in living cells. Methods The fusion gene of H 2Kb and green fluorescent protein (GFP) was constructed by genetic engineering. RT PCR and Western blot analysis of gene expression; laser confocal fluorescence microscope observation of intracellular fluorescence distribution. Results RT PCR and Western blot analysis showed that the fusion gene was correctly expressed. Confocal laser scanning microscopy showed that the Kb GFP fusion molecule was expressed on the cell membrane surface as the native Kb molecule, and the extracellular region was recognized by the Kb molecule-specific monoclonal antibody, indicating that the Kb GFP fusion molecule was correctly folded in the cell And assembly. The same results were obtained both transiently and stably expressing the Kb GFP fusion gene. Conclusion Kb GFP fusion protein has the autofluorescence characteristics of GFP, and does not affect the correct expression of Kb molecules in the cells.