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目的观察氢化可的松与辛伐他汀对高脂血症模型大鼠动脉核转录因子-κB(NF-κB)、血管细胞间粘附因子-1(ICAM-1)含量的影响,探讨其防治动脉粥样硬化(AS)的机制。方法将普通级SD大鼠随机分为空白组,高脂组,氢化可的松高、低剂量组和辛伐他汀组。空白组饲喂基础饲料,其他组饲喂造模饲料并给予相应药物,16周后采用放射免疫法检测血清中三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量;制作主动脉常规及免疫组化切片,观察内膜、肌层厚度及NF-κB、ICAM-1阳性细胞比例。结果氢化可的松高、低剂量组,辛伐他汀组主动脉内膜及肌层厚度明显薄于高脂组,厚于空白组,其NF-κB、ICAM-1阳性细胞率显著低于高脂组,高于空白组。结论氢化可的松、辛伐他汀有抗AS作用,其机制可能通过抑制NF-κB激活和降低ICAM-1分泌有关。
Objective To observe the effect of hydrocortisone and simvastatin on the content of nuclear factor-κB and ICAM-1 in the artery of hyperlipidemia model rats, Mechanisms of atherosclerosis (AS). Methods Normal SD rats were randomly divided into blank group, high fat group, hydrocortisone high and low dose groups and simvastatin group. The blank group was fed with basal diet, and the other groups were fed with the model diet and the corresponding drugs. After 16 weeks, the levels of TG, TC, LDL- C) content. Aortic routine and immunohistochemical sections were made. The thickness of intima and muscular layer and the ratio of NF-κB and ICAM-1 positive cells were observed. Results The thickness of aortic intima and muscular layer in high, low dose hydrocortisone and simvastatin groups was significantly lower than that in high fat group and thicker than blank group, and the positive rates of NF-κB and ICAM-1 were significantly lower than those in high fat group Lipid group, higher than the blank group. Conclusion Hydrocortisone and simvastatin have an anti-AS effect, which may be related to the inhibition of NF-κB activation and ICAM-1 secretion.