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本文报道应用酶联A蛋白ELISA法检测老年前期及老年期心血管病患者血清抗DNA抗体的结果。以酶标仪492nmOD值大于0.14为阳性阈值,同时做免疫荧光法对照,检测了40例心血管病人、22例非心血管非免疫病患者及60份正常人血清。结果显示老年前期及老年期心血管病人抗DNA抗体有较高阳性率,与非心血管病非免疫病及正常人有显著差异。提示老年前期及老年期心血管疾病有些可能与免疫特别是自身免疫有一定关系。
This article reports the enzyme-linked immunosorbent assay (ELISA) for the detection of serum anti-DNA antibodies in pre-elderly and elderly patients with cardiovascular disease. The OD value of 492 nm was above 0.14, and the immunofluorescence method was used as the control. The serum levels of 40 cardiovascular patients, 22 non-cardiovascular non-immune patients and 60 normal individuals were detected. The results showed that pre-elderly and elderly patients with cardiovascular anti-DNA antibodies have a higher positive rate, and non-cardiovascular disease and non-immune disease were significantly different. Prompted pre-senile and elderly cardiovascular disease may be some immune and especially autoimmune have a certain relationship.