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克隆拟南芥LURP1基因上游1 515 bp的启动子调控序列并命名为AtLURP1p,将其与GUS报告基因融合,构建成植物表达载体,分别遗传转化烟草和水稻,获得LURP1p::GUS的烟草和水稻转基因植株及其相应的T2代株系,分别研究LURP1p对水稻稻瘟病、辣椒青枯病侵染及SA、MeJA、ABA等几种重要的植物激素信号分子处理的应答。结果表明:(1)转基因烟草和水稻在几种激素,包括SA、MeJA、ABA的诱导处理下,GUS基因均可以被诱导表达;(2)转基因烟草在细菌性病菌青枯病的侵染下,GUS可被诱导表达并表现出持续表达的趋势,转基因水稻在稻瘟病的侵染下,其GUS基因也被诱导表达,并表现出后期持续上调的趋势。这些研究结果表明,拟南芥LURP1的应答逆境信号通路也存在于烟草和水稻等植物,该启动子可用作诱导型启动子广泛地应用于不同植物抗病基因工程。
Cloning of the upstream of LURP1 gene of Arabidopsis 1 515 bp promoter sequence and named AtLURP1p, and its fusion with the GUS reporter gene to construct a plant expression vector, tobacco and rice were genetically transformed to obtain LURP1p :: GUS tobacco and rice Transgenic plants and their corresponding T2 generation lines were used to investigate the response of LURP1p to several important phytohormones such as rice blast, bacterial wilt infection and SA, MeJA and ABA, respectively. The results showed that: (1) GUS gene can be induced to express in transgenic tobacco and rice under the induction of several hormones, including SA, MeJA and ABA; (2) transgenic tobacco plants were infected with bacterial wilt , GUS could be induced to express and show the trend of sustained expression. The GUS gene of transgenic rice was also induced to express under the infection of rice blast, and showed the tendency of continuing up-regulation in later period. These results indicate that Arabidopsis LURP1 also exists in plants such as tobacco and rice in response to stress signaling pathways. The promoter can be used as an inducible promoter widely in plant disease resistance.