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以紫花苜蓿为实验材料,分别用不同浓度的外源甘氨酸(0、100、200、400、500μmol/L)处理苜蓿幼苗,研究外源甘氨酸对苜蓿幼苗的生长和生理特性的影响.结果表明,不同浓度的外源甘氨酸处理幼苗不仅显著促进了苜蓿幼苗的生长,如幼苗的株高和根的增长以及叶绿素含量的增加,而且提高了苜蓿幼苗超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性,增加了幼苗的抗氧化能力,如丙二醛(MDA)含量降低.但施用500μmol/L的外源甘氨酸相对于施用400μmol/L的外源甘氨酸来说,苜蓿幼苗的SOD和CAT的活性相对降低,MDA的含量相对升高;幼苗的生长相对受到抑制,叶绿素的含量相对降低.与对照组相比,500μmol/L的外源甘氨酸处理仍然增强了苜蓿幼苗的SOD和CAT的活性,提高了苜蓿幼苗的抗氧化能力,降低了MDA的含量.为了提高苜蓿的产量和抗氧化能力,以喷洒400μmol/L的外源甘氨酸效果最佳.
The effects of exogenous glycine on the growth and physiological characteristics of Medicago sativa seedlings were studied by using alfalfa as the experimental material and alfalfa seedlings with different concentrations of exogenous glycine (0, 100, 200, 400 and 500 μmol / L) Different concentrations of exogenous glycine treatment of seedlings not only significantly promoted the growth of alfalfa seedlings, such as seedling height and root growth and chlorophyll content increased, but also increased alfalfa seedlings superoxide dismutase (SOD) and catalase (CAT) increased the antioxidative ability of seedlings, such as the decrease of malondialdehyde (MDA) content. However, the application of 500μmol / L exogenous glycine compared with 400μmol / L exogenous glycine, alfalfa seedlings SOD The activity of CAT and the content of MDA were relatively increased.The growth of seedlings was relatively inhibited and the content of chlorophyll was relatively decreased.Compared with the control group, exogenous glycine at 500μmol / L still enhanced the activities of SOD and CAT in alfalfa seedlings , Increased the antioxidant capacity of alfalfa seedlings and reduced the content of MDA.In order to improve the yield and antioxidant capacity of alfalfa, spraying exogenous glycine at 400μmol / L was the best.