Objective:To investigate whether ginsenoside-Rb1(Gs-Rb1) inhibits the apoptosis of hypoxia cardiomyocytes by up-regulating apelin-APJ system and whether the system is affected by hypoxia-induced factor 1α(Hif-1α).Methods:Neonatal rat cardiomyocytes were randomly divided into 6 groups:a control group,a simple CoCl_2 group,a simple Gs-Rb1 group,a CoCl_2 and Gs-Rb1 hypoxia group,a CoCl_2 and3-(5’-hydroxymethyl-2’-furyl)-1-benzylindazole(YC-1) group,a CoCl_2 and YC-1 group and a Gs-Rb1 group,in which YC-1 inhibits the synthesis and accelerates the degradation of Hif-1α.The concentration of CoCl_2,Gs-Rb1 and YC-1 was 500 μmol/L,200 μ mol/L and 5 μ mol/L,respectively;the apoptosis ratio was analyzed with a flow cytometer;and apelin,APJ and Hif-1α were assayed with immunocytochemistry,Western blot assays and reverse transcription polymerase chain reaction(RT-PCR).Results:(1) The anti-apoptosis effect of Gs-Rb1 on hypoxia cardiomyocytes was significantly inhibited by YC-1;(2) Hypoxia significantly up-graded the expression of mRNA and protein of apelin;this effect was further reinforced by Gs-Rb1 and significantly inhibited by YC-1;(3) Gs-Rb1 further strengthened the expression of APJ mRNA and APJ proteins once hypoxia occurred,which was significantly inhibited by YC-1;(4) Gs-Rb1 significantly increased the expression of Hif-1α,which was completely abolished by YC-1;(5) There was a negative relationship between AR and apelin(or APJ,including mRNA and protein),a positive correlation between apelin(or APJ) protein and Hif-1α protein,in hypoxia cardiomyocytes.Conclusion:The apelin-APJ system plays an important role in the anti-apoptosis effect of GsRb1 on hypoxia neonatal cardiomyocytes,which was partly adjusted by Hif-1α. Objective: To investigate whether ginsenoside-Rb1 (Gs-Rb1) inhibits the apoptosis of hypoxia cardiomyocytes by up-regulating apelin-APJ system and whether the system is affected by hypoxia-induced factor 1α (Hif-1α). Methods: Neonatal rat cardiomyocytes were randomly divided into 6 groups: a control group, a simple CoCl_2 group, a simple Gs-Rb1 group, a CoCl_2 and Gs-Rb1 hypoxia group, a CoCl_2 and 3- (5’-hydroxymethyl-2’-furyl) benzylindazole (YC-1) group, a CoCl_2 and YC-1 group and a Gs-Rb1 group, which inhibits the synthesis and accelerates the degradation of Hif-1α.The concentration of CoCl_2, Gs-Rb1 and YC- 1 was 500 μmol / L, 200 μmol / L and 5 μmol / L, respectively; and the apoptosis ratio was analyzed with a flow cytometer; and apelin, APJ and Hif-1α were assayed with immunocytochemistry, Western blot assays and reverse transcription Results: (1) The anti-apoptosis effect of Gs-Rb1 on hypoxia cardiomyocytes was significantly inhibited by YC-1; (2) Hypoxia si gnificantly up-graded the expression of mRNA and protein of apelin; this effect was further reinforced by Gs-Rb1 and significantly inhibited by YC-1; (3) Gs-Rb1 further strengthened the expression of APJ mRNA and APJ proteins once hypoxia occurred, which was significantly inhibited by YC-1; (4) Gs-Rb1 significantly increased the expression of Hif-1α, which was completely abolished by YC-1; (5) There was a negative relationship between AR and apelin (or APJ, including mRNA and protein), a positive correlation between apelin (or APJ) protein and Hif-1 alpha protein, in hypoxia cardiomyocytes. Conlusion: The apelin-APJ system plays an important role in the anti-apoptosis effect of GsRb1 on hypoxia neonatal cardiomyocytes, which was partly adjusted by Hif-1α.