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从临床医学及病理学上研究汞化合物的作用,已有许多报道。可是,汞化合物进入机体后,通过何种机制妨碍生物机能的报道却不多见。本文用小鼠神经胚胎瘤和神经胶质瘤细胞研究甲基汞和无机汞对这些细胞DNA合成及DNA前身物转运的影响。实验用小鼠神经胶质瘤细胞(SR-CDF DBT)和神经胚胎瘤细胞C-1300(N-18系),分别于含有10%胎牛血清最低必须培养基和Dulbecco’s修改的最低必须培养基中(10%CO_2,36.5℃)培养1～2天。然后将2.0ml磷
Much has been reported about the role of mercury compounds in clinical medicine and pathology. However, it is not uncommon to find reports on the mechanisms by which mechanisms inhibit the biological function of mercury compounds when they enter the body. In this study, mouse embryonic neuroblastoma and glioma cells were used to study the effects of methylmercury and inorganic mercury on the DNA synthesis and the transport of DNA precursors in these cells. Experimental mouse glioma cells (SR-CDF DBT) and neuroblastoma cells C-1300 (line N-18) were cultured in minimal essential medium containing 10% fetal bovine serum minimal essential medium and Dulbecco’s modified Medium (10% CO_2, 36.5 ℃) for 1 to 2 days. Then 2.0 ml of phosphorus