论文部分内容阅读
为提高单核苷酸多态性检测的通量,引入多重嵌合引物PCR和毛细管电泳对四引物扩增受阻突变体系PCR进行改进.针对乳腺癌位点rs4784227(C>T),rs1219648(G>A)和rs3803662(T>C)设计特异性嵌合引物,经一次PCR扩增后,通过毛细管电泳分析产物长度,同时确定3个位点的基因型.70份全血和口腔拭子样本,电泳结果均与测序一致,实现成功分型.本方法仅需一次PCR和一次毛细管电泳即可获得3个位点的分型结果,操作简单、快速准确.
In order to improve the throughput of single nucleotide polymorphism (SNP) detection, the multiplex PCR and capillary electrophoresis were introduced to improve the PCR of the four-primer amplification-blocked mutant.According to rs4784227 (C> T), rs1219648 > A) and rs3803662 (T> C). After a single PCR amplification, the length of the product was analyzed by capillary electrophoresis and the genotypes of the three loci were determined.70 samples of whole blood and buccal swab , Electrophoresis results are consistent with the sequencing, to achieve successful typing.This method only needs a single PCR and a capillary electrophoresis to obtain the typing results of the three loci, the operation is simple, fast and accurate.