论文部分内容阅读
采用离体接种法研究了不同苹果品种被轮纹病菌(Physalospora piricola Nose)侵染后苯丙氨酸解氨酶(PAL)活性以及木质素和绿原酸含量的变化及其与抗病性的关系。接种6d 后各品种(系)枝条的 PAL 活性迅速提高,两天内提高几百倍,果实的 PAL 活性也提高很多;伴随PAL 酶活性的变化,各品种果实、枝条的绿原酸、木质素含量也发生明显的变化,但抗病品种(系)与感病品种差异并不显著。结果表明苹果对轮纹病菌的抗性与苯丙酸类代谢的定速酶 PAL 有关,并通过其代谢产物木质索、绿原酸得到表现,但 PAL 酶活性并不宜作为苹果抗轮纹病生理指标。
The in vitro inoculation method was used to study the changes of PAL activity and lignin and chlorogenic acid contents in different apple cultivars infected with Physalospora piricola Nose and their relationships with disease resistance relationship. After 6 days of inoculation, the PAL activity of the branches of various varieties (lines) increased rapidly and increased hundreds times in two days, and the PAL activity of fruits also increased a lot. With the changes of PAL enzyme activity, the contents of chlorogenic acid and lignin in fruits and branches Significant changes also occurred, but the difference between resistant varieties (lines) and susceptible varieties was not significant. The results showed that the resistance of apple to R.rubrio was related to the rate fixing enzyme PAL of benzenepropanoid metabolism and was expressed by its metabolites xylem and chlorogenic acid. However, the activity of PAL enzyme should not be used as anti-ring-spring physiology index.