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目的 :运用Lowicry1K4M低温包埋及免疫电镜法对甲状腺滤泡细胞源性肿瘤及瘤样病变中甲状腺球蛋白 (Tg)、癌基因蛋白 (Ret)、黏糖蛋白 1(Muc 1)和Galectin 3(Gal 3)基因蛋白产物的表达进行检测。 方法 :对 18例患者手术切除的甲状腺标本进行低温包埋和免疫电镜研究。 结果 :良、恶性甲状腺滤泡上皮细胞的细胞质中胶质小滴、粗面内质网、高尔基复合体及近滤泡的吞饮泡、滤泡腔中有Tg胶体金颗粒 ;本组 9例良性病变 (甲状腺腺瘤和结节性甲状腺肿 )标本Ret、Muc 1和Gal 3三种标记均阴性 ,乳头状癌和滤泡性癌标本的Ret标记可见癌细胞的细胞质中核膜外粗面内质网的核糖体上、粗面内质网腔内及细胞质小泡中有金颗粒的积聚 ,而线粒体、胶质小滴、细胞核、滤泡腔中为阴性 ;Muc 1金颗粒见于粗面内质网、细胞质小泡和细胞膜下 ,细胞核、线粒体、胶质小滴、滤泡腔为阴性 ;Gal 3金颗粒分布较广 ,细胞核常染色质区多见 ,核膜下、粗面内质网、细胞质小泡以及胶质小滴的外周质膜下亦有分布 ,滤泡腔为阴性。 结论 :Ret、Muc 1和Gal 3这三种甲状腺恶性肿瘤标记物亚细胞定位的不完全一致性反映了它们在细胞生物学功能方面可能有一定的差异
OBJECTIVE: To detect the expression of thyroglobulin (Tg), oncoprotein (Ret), mucoprotein 1 (Muc 1) and Galectin 3 (Th1) in thyroid follicular cell carcinoma and tumor-like lesions by Lowicry1K4M cryogenic embedding and immunoelectron microscopy Gal 3) gene protein product. Methods: Thirteen thyroidectomized specimens were cryo-embedded and immunoelectron microscopically studied. Results: Glial droplets, rough endoplasmic reticulum, Golgi complex in the cytoplasm of benign and malignant thyroid follicular epithelial cells, swallowed vesicles near the follicles and Tg colloidal gold particles in the follicular cavity. Nine cases Ret, Muc 1 and Gal 3 were negative in benign lesions (thyroid adenoma and nodular goiter), and Ret markers in papillary carcinoma and follicular carcinoma were seen in the outer nuclear envelope of cancer cells The accumulation of gold particles in the cytoplasm of the ribosome, rough endoplasmic reticulum and cytoplasmic vesicles, but negative in mitochondria, glial droplets, nucleus, and follicular cavity; Muc 1 gold particles found in the rough surface The cytoplasm, cytoplasmic vesicles and cell membrane, nucleus, mitochondria, glial droplets and follicular cavity were negative. The gold particles of Gal 3 were widely distributed, the areas of nuclear chromatin were common, and the nucleus, rough endoplasmic reticulum , Cytoplasmic vesicles and glial droplets under the peripheral plasma membrane are also distributed, follicular cavity is negative. CONCLUSIONS: Incompatibility of subcellular localization of Ret, Muc 1 and Gal 3, three thyroid malignant tumor markers, suggests that they may have some differences in cellular biological function