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目的探讨酪氨酸激酶受体阳性(Tie2+)的单核细胞(Tie2+-U937)对裸鼠移植瘤生长和微血管、淋巴管生成的作用。方法 2013年1月至2014年2月在上海交通大学医学院构建裸鼠卵巢肿瘤皮下和原位模型,转染Tie2+-U937和NC-U937,分别将转染了荧光的人卵巢癌细胞系(SKOV3ip1-luc)与Tie2+-U937或者NC-U937按1:10比例混匀,注入裸鼠皮下和卵巢包膜下,单独的SKOV3作为阴性对照组,观察裸鼠成瘤时间和肿瘤大小;免疫组化检测肿瘤微血管和淋巴管的表达。结果 Tie2+-U937能够促进裸鼠皮下和原位移植瘤生长。皮下成瘤模型:注射后第8、21天Tie2组肿瘤总荧光强度值大于其他两组(P<0.05)。裸鼠于21d处死并称其肿瘤重量,Tie2组(0.43±0.16)g,U937组(0.18±0.04)g,Control组(0.13±0.08)g,Tie2肿瘤重量较其他两组重(P<0.05);Tie2组免疫组化CD31的表达明显大于其余两组(P<0.05);原位模型:注射后第14、35天Tie2组肿瘤总荧光强度值大于其余两组(P<0.05);Tie2组免疫组化CD31和LYVE-1的表达明显大于其余两组(P<0.05)。结论 Tie2+单核细胞能够促进卵巢肿瘤裸鼠皮下、原位移植瘤的生长,并且其促进肿瘤增殖的作用与微血管和淋巴管生成有关。
Objective To investigate the effects of Tie2 + -mono-monocyte (Tie2 + -U937) on the growth, microvascular and lymphangiogenesis of transplanted xenografts in nude mice. Methods The subcutaneous and in situ models of nude mice ovarian tumors were constructed from January 2013 to February 2014 in Shanghai Jiaotong University School of Medicine. Tie2 + -U937 and NC-U937 were transfected into human ovarian cancer cell line ( SKOV3ip1-luc) and Tie2 + -U937 or NC-U937 were injected into nude mice subcutaneously and ovarianly under the capsule and SKOV3 alone as a negative control group to observe the tumorigenic time and size of the tumor in nude mice. Immunohistochemistry To detect the expression of tumor microvessels and lymphatic vessels. Results Tie2 + -U937 can promote subcutaneous and orthotopic transplantation tumor growth in nude mice. Subcutaneous tumorigenicity model: The total fluorescence intensity of Tie2 group was higher than the other two groups on the 8th and 21st day after injection (P <0.05). Tie2 (0.43 ± 0.16) g, U937 (0.18 ± 0.04) g, Control (0.13 ± 0.08) g and Tie2 tumor weights were heavier than those in the other two groups (P <0.05) ). The expression of CD31 in Tie2 group was significantly higher than that in the other two groups (P <0.05). In situ model: The total fluorescence intensity of Tie2 group was higher than the other two groups on the 14th and the 35th day after injection (P <0.05) The expression of CD31 and LYVE-1 in the group of immunohistochemistry was significantly higher than that of the other two groups (P <0.05). Conclusion Tie2 + monocytes promote the growth of subcutaneously and orthotopically transplanted ovarian tumor in nude mice, and its role in promoting tumor proliferation is related to microvascular and lymphangiogenesis.