目的与方法 :为了阐明P5 3蛋白在人肝癌细胞中表达及临床意义 ,应用了免疫组织化学染色方法检测肝细胞癌P5 3蛋白表达情况。结果 :49% (36 / 74)的病例显示有P5 3蛋白的表达 ,低分化癌的P5 3蛋白表达率显著高于高、中分化癌 (χ2 检验P <0 0 5 )。结论 :这些资料显示 :导致P5 3蛋白异常表达的P5 3基因突变是肝细胞癌的一种常见的分子结构改变 ,是评价肝细胞癌预后有价值的一个生物学指标 Objective and Methods: To elucidate the expression and clinical significance of P53 protein in human hepatoma cells, immunohistochemical staining was used to detect the expression of P53 protein in hepatocellular carcinoma. Results: The expression of P53 protein was detected in 49% (36/74) cases. The P5 3 protein expression rate in poorly differentiated carcinoma was significantly higher than that in high and moderately differentiated carcinoma (P<0.05). Conclusion : These data show that the P53 gene mutation that causes the abnormal expression of P53 protein is a common molecular structure change of hepatocellular carcinoma and it is a valuable biological indicator to evaluate the prognosis of hepatocellular carcinoma.