以胶体铁（ＣＩ）、核固红（ＮＦＲ）为染料，以正常和病变肾组织为标本，在传统的单纯ＣＩ染色的基础上复染ＮＦＲ，光镜下比较两种方法的染色结果并观察正常和疾病状态下肾脏负电荷（ＲＮＣ）的分布规律及病理改变。结果表明：改良法不仅弥补了传统法之不足，而且使受染组织着色清晰稳定、颜色对比鲜明。既能准确地反应ＲＮＣ的分布及病理变化，又能清楚地显示肾脏的多种病理改变，尤其对某些病变的染色具有独到之处。从而为ＲＮＣ的病变提供了新的有效的研究方法 NFR was used as a dye to stain NFR in normal and diseased kidney tissues. NFR was stained on the basis of simple CI staining. The staining results of two methods were compared under light microscope The distribution and pathological changes of renal negative charge (RNC) under normal and disease states. The results showed that the modified method not only made up for the deficiencies of the traditional method, but also made the infected tissue clear and stable with distinct color contrast. RNC can not only accurately reflect the distribution and pathological changes, but also clearly shows a variety of pathological changes in the kidney, especially for some lesions with unique features. Thus providing a new and effective research method for the pathological changes of RNC