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目的:建立HPLC法测定芬布芬血浓度。方法:以卡马西平为内标,色谱柱为Hypersil C_(18)柱(200mm×4．6mm,5μm);流速:1．2ml·min~(-1);柱温:45℃;流动相:0．02mol·L~(-1)磷酸二氢钠溶液(含3%三乙胺,用1mol·L~(-1)磷酸溶液调pH 3．00):甲醇=43:57,紫外检测波长284nm。结果:芬布芬的血浓度在0．2～10．0mg·L~(-1)范围内与峰面积比呈良好的线性关系,定量下限为0．2mg·L~(-1)。方法的平均回收率为101．44%±2．3%,内、日间RSD均小于10%。结论:该方法简便、准确,为芬布芬的血药浓度的检测提供了较好的分析方法。
Objective: To establish a HPLC method for the determination of fenbufen blood concentration. Methods: Carbamazepine was used as internal standard. The column was Hypersil C 18 column (200 mm × 4.6 mm, 5 μm), the flow rate was 1.2 ml · min -1, the column temperature was 45 ℃, the mobile phase : 0.02mol·L -1 sodium dihydrogen phosphate solution (containing 3% triethylamine, pH = 3.00 with 1mol·L -1 phosphoric acid solution): methanol = 43:57, UV detection Wavelength 284nm. Results: The blood concentration of fenbufen showed a good linear relationship with the peak area in the range of 0.2-10.0 mg · L -1 with the lower limit of quantitation of 0.2 mg · L -1. The average recovery rate of the method was 101.44% ± 2.3%, RSD of intra-day and inter-day were less than 10%. Conclusion: The method is simple and accurate, and provides a good method for the determination of fenbufen.