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目的 观察不同年龄段人骨髓间充质干细胞 (humanmesenchymalstemcells ,hMSCs)体外生长、增殖的影响。方法 使用密度梯度离心法分离不同年龄人骨髓MSCs进行培养 ,保留贴壁细胞传代 ,观察细胞生长情况 ,检测其增殖活性、细胞周期。结果 低龄hMSCs较高龄hMSCs体外生长快、MTT活性高以及增殖期比例大。结论 hMSCs的增殖能力和活性随着年龄的增加而降低 ,建立其增殖参数可满足组织工程种子细胞在临床应用中不同患者的要求。
Objective To observe the effects of human bone marrow mesenchymal stem cells (hMSCs) on the growth and proliferation in vitro. Methods Human bone marrow MSCs from different ages were isolated by density gradient centrifugation. The passage of adherent cells was preserved. The growth of MSCs was observed. The proliferative activity and cell cycle were detected. Results The hMSCs of younger age grew faster, the activity of MTT was higher and the proportion of proliferative phase was higher than that of hMSCs. Conclusion The proliferation and activity of hMSCs decrease with increasing age. Establishing their proliferation parameters can meet the requirements of different patients in tissue engineering seed cells in clinical application.