双低温敏核不育水稻 (96- 5- 2 S)干种子通过 350 Gy60 Coγ-射线直接辐射 ,处理后的 M1代在育性转换敏感期让其感受自然低温而自交繁殖 .M2 代单本或双本密插 (1 0× 1 3.33cm) ,从 M2 代 2 5万株群体中 ,筛选出 7株长穗颈双低温敏核不育株 .它的成功选育 ,解决了我国两系杂交水稻的制种中不育系育性易出现反复和九二 Ο施用量大的两大技术难题 The seeds of double-temperature-sensitive genic male sterile rice (96- 5-2S) were directly irradiated by 350 Gy60Coγ-rays, and the treated M1 generation self-pollinated in the fertility-switching sensitive period. Seven or two long-necked genic male sterile lines were screened out from 25 000 lines of M2 generation in this or both genotypes (1 0 × 1 3.33cm) Department of hybrid rice seed production sterile line fertility prone to repeated and 1992 two large application of two major technical problems