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目的:了解金华市城区、郊区、农村和山区四个不同区域淡色库蚊对6种常用杀虫剂的抗药性水平,为研究蚊虫抗性产生机制及科学合理使用杀虫剂提供依据。方法:采用药液浸渍法,测定4龄期幼虫半数致死浓度(LC50)。结果:金华市各区域淡色库蚊对氯菊酯抗性倍数最高,均大于10倍,属中抗以上水平;对仲丁威和胺菊酯的抗性倍数较低,均小于4倍,属低抗水平。四个区域中,农村淡色库蚊对各种杀虫剂的抗性最高,其中对氯菊酯、高效氯氰菊酯和溴氰菊酯的抗性倍数均大于10倍。金华市不同区域间自然种群的抗性比较差别无统计学意义(F=0.583,P=0.678);自然种群对不同杀虫剂间抗性发展水平表现不一致,经方差分析差别有统计学意义(F=9.136,P=0.000)。结论:金华市淡色库蚊对6种常用杀虫剂产生了不同程度的抗性,对氯菊酯最高,对胺菊酯、仲丁威普遍敏感,应加强对菊酯类杀虫剂的抗性监测及科学合理使用。
OBJECTIVE: To understand the resistance level of Culex pipiens pallens to six common insecticides in four different districts of Jinhua city, suburban areas, rural areas and mountainous areas, and to provide basis for studying the mechanism of mosquito resistance and using pesticides scientifically and rationally. Methods: The half life lethal concentration (LC50) of 4th instar larvae was determined by liquid immersion method. Results: The resistance fold of Culex pipiens pallens was the highest in Jinhua city, all of which were more than 10 times higher than that of Chrysanthemum, which was less than 4 times of that of Zhong Ding Wei and Tetramethrin Low anti-level. Among the four regions, Culex pipiens pallens had the highest resistance to various insecticides, and the resistance rates to permethrin, beta-cypermethrin and deltamethrin were all more than 10 times higher. There were no significant differences in the resistance of natural populations between different regions in Jinhua City (F = 0.583, P = 0.678). The natural populations showed different levels of resistance development among different insecticides. The variance analysis showed significant difference F = 9.136, P = 0.000). CONCLUSION: Culex pipiens pallens in Jinhua City have different levels of resistance to six commonly used insecticides, with the highest levels of permethrin and the most sensitive to tetramethrin and sec-butyl. The resistance to pyrethroid insecticides should be strengthened Sexual monitoring and scientific and rational use.