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目的探讨建立局部性家兔肠系膜静脉血栓(MVT)模型的方法。方法于2008年1月,将36只家兔随机均分为3组,肠系膜前静脉局部阻断加静脉分支注射凝血酶,A组80U/mL,B组40U/mL,C组注射生理盐水1mL。观测血栓形成时间、范围和周围静脉血D-二聚体(DD)变化。结果 A、B两组均可建立控制范围内稳定MVT模型,血栓形成时间分别为(15.6±2.0)min和(22.3±2.5)min,两组比较有统计学意义(P<0.001);术前A、B两组DD为(68.4±5.7)ng/mL、(72.7±6.4)ng/mL,血栓形成后为(209.0±24.0)ng/mL、(215.4±17.6)ng/mL,组内比较有统计学意义(P<0.001);C组不形成血栓。结论局部静脉阻断加凝血酶注射法可建立稳定和范围可控的MVT模型,建模前后血浆DD值有一定实验参考价值。
Objective To explore a method to establish a model of local rabbit mesenteric vein thrombosis (MVT). Methods In January 2008, 36 rabbits were randomly divided into 3 groups. Local anterior mesenteric vein occlusion plus intravenous injection of thrombin in group A was 80U / mL, group B was 40U / mL, group C was injected with normal saline 1mL . Thrombogenic time, extent and peripheral venous blood D-dimer (DD) changes were observed. Results A stable MVT model was established in both control and control groups. The time to thrombosis was (15.6 ± 2.0) min and (22.3 ± 2.5) min, respectively, with significant difference between the two groups (P <0.001) The DD of group A and group B were (68.4 ± 5.7) ng / mL and (72.7 ± 6.4) ng / mL respectively, and were 209.0 ± 24.0 ng / mL and 215.4 ± 17.6 ng / mL after thrombosis There was statistical significance (P <0.001); C group did not form thrombus. Conclusion Local venous occlusion plus thrombin injection can establish a stable and controlled range of MVT model, the value of plasma DD before and after modeling has some experimental value.