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研究了几种淬灭剂对皖南尖吻蝮蛇蛇毒出血毒素Ⅲ（ＡａＨⅢ）内源荧光的影响。溴代琥珀酰亚胺（ＮＢＳ）法测得每个ＡａＨⅢ分子中含有３个色氨酸（Ｔｒｐ）残基。实验得到丙烯酰胺（Ａｃｒ）和Ｉ－的碰撞淬灭常数Ｋｓｖ分别为３８．３和４．２７Ｌ／ｍｏｌ，ｆａ分别为０．９６和０．９９，Ａｃｒ和Ｉ－均可以淬灭ＡａＨⅢ分子中几乎全部Ｔｒｐ残基的荧光，初步验证Ａｃｒ与ＡａＨⅢ分子间无明显的相互作用。利用荧光淬灭方法，结合ＡａＨⅢ的荧光发射光谱可以推断，ＡａＨⅢ中的Ｔｒｐ残基较大程度位于分子表面的亲水区。
The effects of several quenchers on endogenous fluorescence of AaHⅢ in southern Anhui snake venom were studied. Bromosuccinimide (NBS) assay contains 3 tryptophan (Trp) residues per AaH III molecule. The experimental results showed that the critical quenching constants Ksv of acrylamide (Acr) and I- were 38.3 and 4.27 L / mol, respectively, and fa were 0.96 and 0.99 respectively. Both Acr and I- could quench the AaHIII molecule Fluorescence of almost all Trp residues showed no significant interaction between Acr and AaHIII molecules. Fluorescence quenching method, combined with the fluorescence emission spectrum of AaHIII, we can infer that the Trp residues in AaHIII are largely located in the hydrophilic surface of the molecule.