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目的结合表达谱芯片技术从2型糖尿病肾虚证入手进行研究探索肾虚因素在2型糖尿病发病作用。方法将中医肾虚证作为共性特征与糖尿病结合起来,设立肾虚证糖尿病组与非肾虚证糖尿病组,另设健康对照组为对照,以表达谱芯片研究手段,对其结果通过数据库进行分析,并选择差异明显的部分基因进行PCR检测验证。结果肾虚组与正常对照组相比,上调基因134条,下调1038条,肾虚组与非肾虚组比较,上调基因45条,下调61条。肾虚糖尿病与非肾虚糖尿病差异表达基因通过在GO数据库中搜索发现,主要归于三类:分子功能,细胞组成和生物学过程,涉及通路主要为白细胞内皮迁移过程,溶酶体作用,细胞的紧密连接作用等。荧光定量PCR检测结果初步确定基因CTNNB1、SMURF1、RUNX1T1在肾虚证糖尿病组中的表达水平与非肾虚证糖尿病组相比较,前者较后者具有表达上调的趋势,但差异不显著(P>0.05)。结论糖尿病是一种与机体代谢机能紊乱相关的疾病,肾虚证是糖尿病人群的基础表现证型,肾虚证更多的是体现个体组织细胞结构及蛋白表达的差异表达,在细胞水平上的差异造就了肾虚证的证候。
Objective To investigate the role of kidney deficiency in the pathogenesis of type 2 diabetes mellitus (T2DM) by combining with profiling microarray technology. Methods The combination of traditional Chinese medicine Kidney Deficiency Syndrome and diabetes mellitus was established. Diabetic patients with kidney deficiency syndrome and non-kidney deficiency diabetes mellitus group were established. Another healthy control group was used as a control. The results were analyzed by database and selected Some genes with significant difference were verified by PCR. Results Kidney deficiency group compared with the normal control group, up-regulated 134 genes, down 1038, kidney deficiency group and non-kidney group, up-regulated 45 genes, down 61. Differentially expressed genes of kidney-deficiency and non-kidney-diabetic patients were mainly found in three categories: molecular function, cell composition and biological process, and the pathways involved mainly were leukocyte endothelium migration process, lysosomal function, tight junction of cells Role and so on. Compared with non-kidney-deficiency diabetes mellitus group, the expression of CTNNB1, SMURF1 and RUNX1T1 in kidney-deficiency type diabetes mellitus group was higher than that in the non-kidney-deficiency type diabetes mellitus group (P> 0.05) . Conclusions Diabetes mellitus is a disease associated with metabolic disorder. Kidney deficiency syndrome is the basic manifestation syndrome in diabetic patients. Kidney deficiency syndrome is the result of differential expression of cell structure and protein expression in individual tissues. The syndrome of kidney deficiency syndrome.