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目的:对FZ06008株、FZ17408株VP1进行基因特性分析。方法:采集福州市2008年6月手足口病人的标本,接种于RD细胞进行病毒分离,采用RT-PCR方法进行核酸鉴定,将VP1基因克隆和序列测定,并利用DNA-STAR软件对VP1基因进行遗传性分析。结果:与标准株B rCr相比,FZ06008和FZ17408的VP1编码区没有出现核苷酸的缺失和插入,氨基酸同源性约为94.6%,核苷酸同源性约为79.1%;与安徽省2008年流行株703813-Fuyang-08和我国深圳2003年流行的SHZH03株相比,氨基酸同源性约为99%~99.3%。结论:FZ06008株和FZ17408株有可能来源于2003年深圳地区EV71大规模流行时的毒株,其进化途径可能与安徽阜阳的标准株703813-Fuyang-08相近。
Objective: To analyze the gene characteristics of VP1 in FZ06008 strain and FZ17408 strain. Methods: The specimens of hand, foot and mouth in Fuzhou were collected and inoculated into RD cells for virus isolation. The nucleic acid was identified by RT-PCR. The VP1 gene was cloned and sequenced. The VP1 gene was amplified by DNA-STAR software Hereditary analysis. Results: Compared with the standard strain B rCr, there were no nucleotide deletions and insertions in the VP1 coding region of FZ06008 and FZ17408, with amino acid homology of about 94.6% and nucleotide homology of about 79.1% Compared with SHZH03 strain popular in Shenzhen in 2003 in China in 2008, 703813-Fuyang-08, the amino acid homology is about 99% -99.3%. Conclusion: The strains FZ06008 and FZ17408 may originate from the strain EV71 in Shenzhen in 2003, and its evolutionary path may be similar to the standard strain 703813-Fuyang-08 in Fuyang of Anhui Province.