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本研究测定了5个石蒜属植物(石蒜、忽地笑、换锦花、中国石蒜和长筒石蒜)鳞茎对–5℃低温的生理响应。结果表明,石蒜的耐寒性较好。在此基础上,以石蒜为试验材料,研究了不同低温胁迫(4、0、–5和–10℃)与露地环境对石蒜植株形态及相关生理指标的影响。结果表明,在形态方面,4和0℃低温处理对石蒜无明显的作用;–5℃处理导致了叶片轻度受冻、萎蔫,鳞茎也表现为轻微的受冻、失水;–10℃处理导致了叶片重度受冻并失水萎蔫,鳞茎死亡;露地处理下,只有叶片出现轻度受冻和失水症状,鳞茎并未受到显著影响。在生理方面,4和0℃处理的各生理指标均无显著性变化;–5℃处理虽对各生理指标有较大影响,但尚可恢复;–10℃持续低温已超过石蒜的低温忍耐极限,导致了叶片中SOD完全失活,鳞茎中可溶性蛋白显著升高等变化,并最终导致了鳞茎完全坏死,植株死亡;露地处理表现优于–10℃处理,表明石蒜对非持续的极限低温忍耐程度更强。
In this study, the physiological responses of 5 Lycoris (Lycoris, Suddenly Laughing, Translucent, Chinese Lycoris and Lycoris) bulb to -5 ℃ were measured. The results show that Lycoris better cold tolerance. On this basis, the effects of different low temperature stress (4, 0, -5 and -10 ℃) and the open field environment on the morphology and related physiological indexes of Lycoris radiata were studied. The results showed that there was no obvious effect on the morphology of Lycoris radiate at 4 ℃ and 0 ℃ in the morphological aspect. The treatment at -5 ℃ led to slight freezing and wilting of the leaves, and the bulb also showed a slight freezing and dehydration. The treatment resulted in the severe freezing of the leaves and the wilting of the wilting water and the death of the bulbs. Under the field treatment, only the leaves were slightly affected by freezing and dehydration, and the bulbs were not significantly affected. Physiologically, no significant changes were observed in physiological parameters at 4 and 0 ℃. Although -5 ℃ treatment had a greater impact on each physiological index, it still recovered; the low temperature of -10 ℃ continued to exceed that of Lycoris radiate Which led to the complete inactivation of SOD in leaves and the significant increase of soluble protein in bulbs, which led to the complete necrosis of bulb and death of plants. The performance of open-field treatment was better than that of -10 ℃, which indicated that the effect of Lycoris radiate on non-sustained limit of low temperature Be patient.