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【目的】在粗山羊草(Aegilops tauschii)中寻找新的抗叶锈病基因,为抗病育种提供新种质。【方法】本研究对抗小麦叶锈病的粗山羊草Y192和感小麦叶锈病的Y2272进行杂交,通过F2代抗叶锈性分离情况确定可能含有的抗叶锈基因数量,应用分离群体分组法(bulked segregation analysis,BSA)筛选D染色体上与抗叶锈性相关的SSR标记,用MapChart软件构建遗传连锁图谱。利用分子辅助鉴定和抗叶锈表型分析推测Y192可能含有的抗叶锈基因。【结果】在接菌04-5-192(THNT)的杂交后代中F1代表现抗病,F2代表现3:1的抗感分离,表明该基因为一个显性抗病基因,将该抗病基因暂命名为LrY192。筛选到的3个SSR标记Wmc245、Xgwm296和Xgwm261与该基因的遗传距离分别为4.1、18.9和26.2 cM。根据连锁标记在小麦微卫星图谱的位置,LrY192被定位在2D染色体上。【结论】综合分析基因所在的染色体位置及抗病特性,认为LrY192是一个新的抗小麦叶锈基因,获得的SSR标记Wmc245可用于分子辅助选择。
【Objective】 The aim of the study was to search for new leaf rust resistance genes in Aegilops tauschii and provide new germplasm for resistance breeding. 【Method】 The present study was conducted to test the relationship between Y222 and Y2272 susceptible to wheat leaf rust and to determine the possible anti-leaf rust genes by the isolation of leaf rust resistance in F2 generation. Bulked segregation segregation analysis (BSA). The SSR markers related to leaf rust resistance on chromosome D were screened. MapChart software was used to construct the genetic linkage map. Using molecular-assisted identification and phenotypic analysis of leaf rust speculated Y192 may contain anti-leaf rust genes. 【Result】 F1 was resistant to disease in F1 progenies of Fusarium graminearum 04-5-192 (THNT), F2 showed a 3: 1 anti-susceptibility segregation, indicating that this gene was a dominant resistance gene. Gene temporarily named LrY192. The genetic distances of the three SSR markers Wmc245, Xgwm296 and Xgwm261 to this gene were 4.1, 18.9 and 26.2 cM, respectively. LrY192 is mapped on the 2D chromosome according to the position of the linkage marker in the wheat microsatellite map. 【Conclusion】 The results showed that LrY192 was a new anti-wheat leaf rust gene, and the obtained SSR marker Wmc245 could be used for molecular-assisted selection.