论文部分内容阅读
目的阐明在广西瑶族地区新发现的两个肝癌高发家族发生肝癌家族聚集性的危险因素。方法以广西瑶族不同地区的两肝癌高发家族成员作为研究对象,应用ELISA法检测研究对象血清中HBV和HCV血清学标志物、采用PCR和RT-PCR技术分别检测HBV DNA和HCV RNA,结合流行病学调查结果进行综合分析。结果两肝癌高发家族成员中HBsAg、HBsAb、HBeAg、HBeAb、HBcAb、HBVDNA、抗-HCV、HCVRNA的阳性率分别为45.0%(9/20)、35.0%(7/20)、15.0%(3/20)、25.0%(5/20)、45.0%(9/20)、25.0%(5/20)、0(0/20)、0(0/20)和9.52%(2/21)、42.9%(9/21)、4.8%(1/21)、4.8%(1/21)、23.8%(5/21)、9.52%(2/21)、0(0/21)、0(0/21)。其中HBsAg在两组间的差别具有统计学意义(P=0.033),A家族以山泉水作为饮用水,B家族十年前以塘水作为生活用水;其余因素差异未见统计学意义。结论瑶族不同地区、不同家族成员发生肝癌家族聚集性的危险因素可以有所不同,HBV感染、饮用塘水等因素均可与遗传因素相互作用,从而导致肝癌家族聚集性的发生。
Objective To elucidate the risk factors of familial hepatocellular carcinoma (HCC) clustering in two newly diagnosed HCC patients in Yao ethnic region of Guangxi province. Methods Serum HBV and HCV serological markers were detected by ELISA. HBV DNA and HCV RNA were detected by PCR and RT-PCR, respectively. Combined with epidemiology Study the results of a comprehensive analysis. Results The positive rates of HBsAg, HBsAb, HBeAg, HBeAb, HBcAb, HBVDNA, anti-HCV and HCVRNA were 45.0% (9/20), 35.0% (7/20) and 15.0% 25.0% (5/20), 0 (0/20), 0 (0/20) and 9.52% (2/21), 42.0 (1/21), 4.8% (1/21), 23.8% (5/21), 9.52% (2/21), 0 (0/21), 0 (0/21) twenty one). Among them, the difference of HBsAg between two groups was statistically significant (P = 0.033). A family took mountain spring water as drinking water, and family B ten years ago used pond water as domestic water. There was no statistical difference between the other factors. Conclusion The risk factors of familial HCC aggregation in different areas and different family members in Yao may be different. Factors such as HBV infection and drinking pond water may interact with genetic factors, leading to the occurrence of familial aggregation of HCC.