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目的探讨先天性人巨细胞病毒(HCMV)感染对肝功能与乙型肝炎疫苗免疫效果的相互影响。方法孕鼠于见栓3~5d腹腔注射HCMV悬液,其仔鼠作为先天性HCMV感染模型,随机分为先天性HCMV感染组(感染组)、更昔洛韦治疗组(治疗组),以健康大鼠的仔鼠作为对照组。各组仔鼠分别于生后1、3、5周接种乙肝疫苗,并于3、5、7、11周心脏采血,采血后2h内应用全自动生化分析仪检测丙氨酸氨基转移酶(ALT)浓度,留取血清应用酶联免疫分析方法检测血清乙肝表面抗体(HBsAb)、透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原(ColⅣ)的浓度。结果3、5、7、11周时感染组ALT浓度分别为(66.31±6.62)、(55.14±8.03)、(55.27±6.50)、(49.85±8.06)IU/L,均高于治疗组与对照组,差异均有统计学意义(P<0.05);3周时治疗组仔鼠的ALT浓度高于对照组(P<0.05)。在各时间点(3、5、7、11周),感染组HBsAb滴度分别为(0.54±0.17)、(1.28±0.66)、(2.45±0.74)、(2.13±0.76)U/ml,均低于治疗组和对照组,差异均有统计学意义(P<0.05),治疗组HBsAb滴度低于对照组,差异有统计学意义(P<0.05)。在各时间点(3、5、7、11周),感染组仔鼠的HA、LN、PCⅢ和ColⅣ的浓度均显著高于治疗组与对照组,差异有统计学意义(P<0.05);3周、5周时治疗组HA、LN、PCⅢ检测结果高于对照组,异有统计学意义(P<0.05)。结论先天性人巨细胞病毒感染可造成肝损伤,影响肝功能,导致机体对乙肝疫苗免疫应答降低;及时治疗,有利于改善肝功能,增强乙肝疫苗的免疫效果。
Objective To investigate the interaction between congenital human cytomegalovirus (HCMV) infection and the immune effect of hepatitis B vaccine. Methods Pregnant mice were intraperitoneally injected with HCMV suspension for 3 to 5 days and their offspring were randomly divided into congenital HCMV infection group (infection group) and ganciclovir treatment group (treatment group) Healthy rats offspring as a control group. All the pups were inoculated with hepatitis B vaccine at 1, 3, and 5 weeks after birth, and the blood samples were taken at the 3rd, 5th, 7th and 11th weeks after the blood was collected. The serum levels of alanine aminotransferase (ALT Serum samples were collected for detection of serum HBsAb, HA, PCⅢ and ColⅣ by enzyme-linked immunosorbent assay (ELISA) concentration. Results The ALT concentrations in infected group were (66.31 ± 6.62), (55.14 ± 8.03), (55.27 ± 6.50) and (49.85 ± 8.06) IU / L at 3, 5, 7 and 11 weeks (P <0.05). At 3 weeks, ALT levels in the offspring of treatment group were higher than those of the control group (P <0.05). The HBsAb titers in infected group were (0.54 ± 0.17), (1.28 ± 0.66), (2.45 ± 0.74) and (2.13 ± 0.76) U / ml at each time point The difference was statistically significant (P <0.05). The titer of HBsAb in the treatment group was lower than that in the control group (P <0.05). At each time point (3, 5, 7, 11 weeks), the concentrations of HA, LN, PC Ⅲ and Col Ⅳ in the offspring of the infected group were significantly higher than those of the treatment group and the control group (P <0.05). The results of HA, LN and PC Ⅲ in the treatment group were higher than those in the control group at 3 and 5 weeks (P <0.05). Conclusion Infection of congenital human cytomegalovirus can cause liver damage, affect liver function, and lead to the body to reduce the immune response to hepatitis B vaccine; timely treatment, is conducive to improving liver function and enhance the immune effect of hepatitis B vaccine.