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目的 :研究前列腺良、恶性病变组织中细胞增殖与细胞凋亡的关系及其生物学意义。方法 :对 36例前列腺癌(Pca)、2 0例前列腺增生 (BPH)和 10例正常前列腺 (NP)的细胞增殖和凋亡情况进行研究。应用免疫组织化学ABC法检测增殖细胞核抗原 (PCNA)来反映细胞增殖活性 (PI) ,用原位末端标记 (TUNEL)的方法反映细胞凋亡 (AI)情况。结果 :PI和AI在前列腺癌组织中的表达明显高于良性前列腺增生 (P <0 0 1) ,PCNA与前列腺癌分级有关 ,随着肿瘤分级增高而增高 ;前列腺增生组织中细胞增殖活性较正常前列腺明显增高 ,但细胞的凋亡率却显著下降。结论 :细胞增殖与细胞凋亡的增加在前列腺癌的发生和发展中起重要作用 ;而前列腺组织细胞增殖的增加和细胞凋亡的减少参与了BPH形成过程。
Objective: To study the relationship between cell proliferation and apoptosis in benign and malignant prostatic lesions and its biological significance. Methods: Proliferation and apoptosis of 36 cases of prostate cancer (Pca), 20 cases of benign prostatic hyperplasia (BPH) and 10 cases of normal prostate (NP) were studied. Immunohistochemical ABC method was used to detect proliferating cell nuclear antigen (PCNA) to reflect the cell proliferative activity (PI), and the apoptosis (AI) was detected by TUNEL. Results: The expression of PI and AI in prostate cancer tissues was significantly higher than that in benign prostatic hyperplasia (P <0.01). PCNA was associated with the grade of prostate cancer and increased with the grade of tumor. The cell proliferative activity in benign prostatic hyperplasia was more than normal Prostate significantly increased, but the rate of apoptosis was significantly decreased. Conclusion: The increase of cell proliferation and apoptosis play an important role in the occurrence and development of prostate cancer. Prostatic hyperplasia and the decrease of apoptosis are involved in the process of BPH formation.