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以甘蓝型油菜野油19号的下胚轴和带柄子叶为外植体,研究其下胚轴和带柄子叶在不同浓度激素配比下的分化率及再生频率的变化。该品系的油菜下胚轴和带柄子叶在1.5 mg/L的2,4-D中预培养4 d后,转入分化培养基中,其愈伤组织形成早,发生快,再生频率和分化频率均较高。其中,下胚轴转入MS+3 mg/L 6-BA+0.1 mg/L NAA培养基中的分化率和再生频率最高,再生频率达到86.67%;带柄子叶外植体的再生频率要比下胚轴的再生频率低,在MS+4 mg/L 6-BA+0.3 mg/L NAA的分化培养基中芽的再生频率为46.67%。本研究初步建立了甘蓝型油菜野油19号的高频率再生体系。
The hypocotyls and cotyledons with the cotyledons of Hanyou No. 19 were used as explants to study the differentiation and regeneration frequency of hypocotyls and cotyledons with different concentrations of hormones. The rape hypocotyls and cotyledons of this strain were pre-cultured in 1.5 mg / L 2,4-D for 4 days and then transferred to differentiation medium. The callus was formed early, rapidly, and the regeneration frequency and differentiation The frequency is higher. Among them, the transformation rate and regeneration frequency of hypocotyl into MS + 3 mg / L 6-BA + 0.1 mg / L NAA medium were the highest, and the regeneration frequency reached 86.67%. The regeneration frequency of explants with cotyledons was The regeneration frequency of hypocotyls was low, and the regeneration frequency of shoots was 46.67% in MS + 4 mg / L 6-BA + 0.3 mg / L NAA differentiation medium. In this study, we initially established a high-frequency regeneration system of Brassica napus No.9 oil.