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10只中国1号小鼠,5只为幼鼠(45天)和5只成鼠(95天),其呼吸道组织标本固定于等渗甲醛-醋酸混合液,分别用甲苯胺蓝和Alcian蓝-藏红染色,以显示肥大细胞。并用其皮肤肥大细胞作对照。结果表明:单位面积呼吸道粘膜的结缔组织肥大细胞(CTMC)数,在鼻腔粘膜显著高于喉、气管和肺。同时,呼吸道肥大细胞随年龄而增加,在成鼠明显多于幼鼠(p<0.05)。用Alcian蓝-藏红染色的成鼠肥大细胞颗粒,其Alclan蓝阳性率高于幼鼠。气管和肺内偶见粘膜型肥大细胞(MMC)。
Ten Chinese 1 mice, 5 were young rats (45 days) and 5 rats (95 days). The airway tissue specimens were fixed in the solution of isotonic formaldehyde - acetic acid mixed with toluidine blue and Alcian blue - Stained red to show mast cells. And with its skin mast cells as a control. The results showed that the number of connective tissue mast cells (CTMCs) per unit area of the respiratory mucosa was significantly higher in the nasal mucosa than in the larynx, trachea and lung. Meanwhile, respiratory tract mast cells increased with age, significantly more in adult rats than in young rats (p <0.05). Alcian blue-stained red staining of adult rat mast cell particles, Alclan blue positive rate higher than the young rats. Tracheal and pulmonary occasionally mucosal mast cells (MMC).