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植物叶片是最主要的光合作用器官.作物叶片生长、发育和衰老的分子机理研究与提高作物产量形成密切相关.利用水稻中花11号经Co60辐射产生的早衰叶突变体分别与南京6号和南京11号杂交的F1及其衍生的F2群体,对早衰叶突变体进行了遗传分析和基因定位.结果表明,该早衰叶突变体是由一隐性核基因psl1控制,利用SSR标记把psl1定位在水稻第2染色体上.利用已经公布的水稻基因组序列,在该基因附近区域发展了34对新的STS标记,对psl1进行了精细定位.以此为基础,构建了覆盖psl1区域的BAC重叠群,并把目标基因定位在一个约48kb的区段上,为最终克隆目标基因奠定了基础.
Plant leaves are the most important photosynthetic organs.Studies on the molecular mechanism of crop leaf growth, development and senescence are closely related to the improvement of crop yield formation.Using rice premature leaf decay mutant produced by Co60 radiation in Zhonghua 11, The F1 progenies from Nanjing 11 and F2 populations derived from them were used to carry out genetic analysis and gene mapping of premature leaf lobe mutants.The results showed that the premature leaf lobe mutant was controlled by a recessive nuclear gene psl1 and the SSR marker was used to locate psl1 On the second chromosome of rice, 34 pairs of new STS markers were developed in the vicinity of this gene and the psl1 was finely mapped using published rice genome sequences. Based on this, a BAC contig covering the psl1 region was constructed , And locating the target gene in a segment of about 48kb, which laid the foundation for the final cloning of the target gene.