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以基因工程抗原取代天然抗原用于人巨细胞病毒（ＨＣＭＶ）感染的诊断，具有广阔的前景。为此，分离ＨＣＭＶ大外膜磷蛋白ｐｐ１５０基因ＯＲＦ３端４３４ｂｐ（编码羧基末端ａａ９４３～ａａ１０４８）的ＤＮＡ片段，导入带有高水平转录启动子Ｐｔｒｃ和六个串联组氨酸的原核表达载体ｐＴｒｃＨｉｓ，在大肠杆菌中得到高效表达，ＳＤＳＰＡＧＥ显示一明显的分子量为１５６ｋＤ的融合蛋白条带，ＬＫＢ激光扫描占菌体总蛋白的５１％以上，并且以可溶性形式存在。表达产物经高效特异性纯化后，ＥＬＩＳＡ结果表明其能够与ＨＣＭＶＩｇＭ阳性血清呈特异反应，为建立新一代ＨＣＭＶＩｇＭ检测试剂盒打下了基础。
The use of genetically engineered antigens instead of natural antigens for human cytomegalovirus (HCMV) infection diagnosis, has broad prospects. For this purpose, a DNA fragment of 434 bp (encoding carboxyl terminal aa943 to aa1048) at the ORF3’end of HCMV large outer membrane phosphoprotein pp150 gene was isolated and introduced into prokaryotic expression vector pTrcHis with a high level of transcription promoter Ptrc and six tandem histidines. Efficient expression in E. coli, SDS-PAGE showed a clear molecular weight of 15 6kD fusion protein bands, LKB laser scan accounted for more than 51% of the total bacterial protein, and exists in soluble form. After the specific and efficient purification of the expressed product, the ELISA result showed that it can react specifically with HCMVIgM positive serum, laying a foundation for establishing a new generation HCMVIgM detection kit.