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采用离体细胞体外孵育法 ,研究反义c erbB2 寡脱氧核苷酸 (antisensec erbB2 ODN)对大鼠黄体细胞hCG诱导的孕酮分泌的影响 ,及其与外源性cAMP和Ca2 +以及蛋白抑制剂放线菌酮 (CYX)之间的关系。结果表明 ,反义c erbB2 以剂量相关方式抑制黄体细胞hCG诱导的孕酮的产生 ,同时使c erbB2 蛋白染色阳性的黄体细胞百分数下降 ,无义tatODN没有相应的作用。10 -4 mol/L的二丁酰cAMP能明显反转反义c erbB2 ODN对孕酮产生和c erbB2 表达的抑制作用 ,钙离子通道阻断剂维拉帕米和蛋白抑制剂CYX对此抑制作用有协同效应。该实验说明c erbB2 参于hCG诱导黄体细胞生孕酮作用
The effect of antisense c erbB2 ODN on hCG-induced progesterone secretion in rat luteal cells was studied by in vitro incubation in vitro. The effects of exogenous cAMP and Ca2 + and protein inhibition Agent cycloheximide (CYX) the relationship between. The results showed that antisense c erbB2 dose-dependently inhibited hCG-induced progesterone production in luteal cells while decreased the percentage of corpus luteum cells stained for c-erbB2 protein, without any effect on tatODN. 10 -4 mol / L of dibutyryl cAMP significantly reversed the inhibitory effect of antisense c erbB2 ODN on progesterone production and c erbB2 expression, which was inhibited by the calcium channel blocker verapamil and protein inhibitor CYX A synergistic effect. This experiment demonstrates that cb erbB2 is involved in hCG-induced progesterone production in luteal cells