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目的建立椎间盘内破裂的动物模型,观察其病理学及行为学表现。方法实验用雄性SD大鼠96只,随机分为3组,每组32只。假手术组(对照组),全麻后取仰卧位,经腹腔正中入路,钝性撕开后腹膜,暴露L_(6～7)椎间盘,逐层缝合。椎间盘轻度破坏组(手术1组),全麻后取仰卧位,经腹腔正中入路,暴露L_(6～7)椎间盘。于椎间盘前正中点以直径0.2mm的特制针头刺入L_(6～7)椎间盘至后侧纤维环下,深度控制为3mm。少许明胶海绵堵塞针口,逐层缝合。关腹前滴入约1ml左右生理盐水。椎间盘重度破坏组(手术2组),全麻后取仰卧位,经腹腔正中入路,暴露L_(6～7)椎间盘。于椎间盘前正中点以直径0.2mm的特制针头刺入L_(6～7)椎间盘至后侧纤维环下,深度控制为3mm。仍以前正中针口为刺入点,与第一针道各成30°角向左右分别穿刺至后外侧纤维环下。结果手术椎间盘内可见由前方至后方的裂隙,后侧的外层纤维环未破裂。术后髓核内纤维组织样细胞透明样变性,与胶冻状物交织在一起,内层纤维软骨细胞变性。椎间盘内破裂模型手术2组的大鼠术后静止不动时间延长、运动时间减少,同假手术组相比,有统计学意义。结论建立了一种椎间盘内破裂的动物模型,并观察了手术间盘的病理及行为学改变过程,该模型可模拟临床上的椎间盘源性下腰痛。
Objective To establish an animal model of intradiscal rupture and observe its pathological and behavioral findings. Methods Ninety-six male Sprague-Dawley rats were randomly divided into 3 groups (32 in each group). The sham-operation group (control group) received supine position after general anesthesia, and the posterior peritoneum was blunt dissected through the middle of the abdominal cavity. The L_ (6 ~ 7) intervertebral discs were exposed and stitched one by one. The lumbar intervertebral disc was slightly damaged (operation group 1). After general anesthesia, the supine position was taken and the L_ (6 ~ 7) disc was exposed through the midclavicular approach. At the midpoint of the anterior disc, a special needle with a diameter of 0.2mm was inserted into the L 6-7 disc into the posterior annulus fibrosus, and the depth was controlled to 3mm. A little gelatin sponge plug pinhole, layer by layer. About 1ml of normal saline drops before closing the abdomen. Severe discectomy group (surgery group 2), supine after general anesthesia, transabdominal approach, exposed L_ (6 ~ 7) intervertebral disc. At the midpoint of the anterior disc, a special needle with a diameter of 0.2mm was inserted into the L 6-7 disc into the posterior annulus fibrosus, and the depth was controlled to 3mm. Still in front of the needle as the piercing point, and the first needle into the 30 ° angle to the left and right puncture to the posterolateral annulus fibrosus. Results There was a fissure from the anterior to the posterior in the operative disc, while the posterior annulus fibrosus was not ruptured. Postoperative nucleus pulposus within the fibrous tissue-like cells degeneration, and jelly intertwined, degeneration of inner fibrous chondrocytes. The intravertebral disc rupture model operation rats in group 2 were immobilized for a longer period of time, the exercise time decreased, compared with the sham operation group, there was statistical significance. Conclusion An animal model of intradiscal rupture was established and the pathological and behavioral changes of the intervertebral disc were observed. The model can simulate the clinical discogenic low back pain.